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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella amazonensis Putative reductase Sama_1230 (Sama_1230) CSB-YP377709STK
CSB-EP377709STK
CSB-BP377709STK
CSB-MP377709STK
CSB-EP377709STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Chaperone protein hscA homolog (hscA), partial CSB-YP377710STK
CSB-EP377710STK
CSB-BP377710STK
CSB-MP377710STK
CSB-EP377710STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Adenylate kinase (adk) CSB-YP377711STK
CSB-EP377711STK
CSB-BP377711STK
CSB-MP377711STK
CSB-EP377711STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0434 protein Sama_1339 (Sama_1339) CSB-YP377712STK
CSB-EP377712STK
CSB-BP377712STK
CSB-MP377712STK
CSB-EP377712STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Biotin synthase (bioB) CSB-YP377713STK
CSB-EP377713STK
CSB-BP377713STK
CSB-MP377713STK
CSB-EP377713STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA ligase (ligA), partial CSB-YP377714STK
CSB-EP377714STK
CSB-BP377714STK
CSB-MP377714STK
CSB-EP377714STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein proQ homolog (proQ) CSB-YP377715STK
CSB-EP377715STK
CSB-BP377715STK
CSB-MP377715STK
CSB-EP377715STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosome modulation factor (rmf) CSB-YP377716STK
CSB-EP377716STK
CSB-BP377716STK
CSB-MP377716STK
CSB-EP377716STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Elongation factor P (efp) CSB-YP377717STK
CSB-EP377717STK
CSB-BP377717STK
CSB-MP377717STK
CSB-EP377717STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP377718STK
CSB-EP377718STK
CSB-BP377718STK
CSB-MP377718STK
CSB-EP377718STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Cytidylate kinase (cmk) CSB-YP377719STK
CSB-EP377719STK
CSB-BP377719STK
CSB-MP377719STK
CSB-EP377719STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Integration host factor subunit beta (ihfB) CSB-YP377720STK
CSB-EP377720STK
CSB-BP377720STK
CSB-MP377720STK
CSB-EP377720STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Outer-membrane lipoprotein carrier protein (lolA) CSB-YP377721STK
CSB-EP377721STK
CSB-BP377721STK
CSB-MP377721STK
CSB-EP377721STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP377722STK
CSB-EP377722STK
CSB-BP377722STK
CSB-MP377722STK
CSB-EP377722STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Electron transport complex protein RnfG (rnfG), partial CSB-YP377723STK
CSB-EP377723STK
CSB-BP377723STK
CSB-MP377723STK
CSB-EP377723STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP377724STK
CSB-EP377724STK
CSB-BP377724STK
CSB-MP377724STK
CSB-EP377724STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA (cmo5U34)-methyltransferase CSB-YP377725STK
CSB-EP377725STK
CSB-BP377725STK
CSB-MP377725STK
CSB-EP377725STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Thiazole synthase (thiG) CSB-YP377726STK
CSB-EP377726STK
CSB-BP377726STK
CSB-MP377726STK
CSB-EP377726STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Fatty acid metabolism regulator protein (fadR) CSB-YP377727STK
CSB-EP377727STK
CSB-BP377727STK
CSB-MP377727STK
CSB-EP377727STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0260 protein Sama_1927 (Sama_1927) CSB-YP377728STK
CSB-EP377728STK
CSB-BP377728STK
CSB-MP377728STK
CSB-EP377728STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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