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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella sp. Cell division topological specificity factor (minE) CSB-YP376209STT
CSB-EP376209STT
CSB-BP376209STT
CSB-MP376209STT
CSB-EP376209STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Na (+)/H (+) antiporter nhaB, partial CSB-YP376210STT
CSB-EP376210STT
CSB-BP376210STT
CSB-MP376210STT
CSB-EP376210STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP376211STT
CSB-EP376211STT
CSB-BP376211STT
CSB-MP376211STT
CSB-EP376211STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP376212STT
CSB-EP376212STT
CSB-BP376212STT
CSB-MP376212STT
CSB-EP376212STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Uracil phosphoribosyltransferase (upp) CSB-YP376213STT
CSB-EP376213STT
CSB-BP376213STT
CSB-MP376213STT
CSB-EP376213STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Phosphate acyltransferase (plsX) CSB-YP376214STT
CSB-EP376214STT
CSB-BP376214STT
CSB-MP376214STT
CSB-EP376214STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0434 protein Sputw3181_2540 (Sputw3181_2540) CSB-YP376215STT
CSB-EP376215STT
CSB-BP376215STT
CSB-MP376215STT
CSB-EP376215STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP376216STT
CSB-EP376216STT
CSB-BP376216STT
CSB-MP376216STT
CSB-EP376216STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Bifunctional protein FolD (folD) CSB-YP376217STT
CSB-EP376217STT
CSB-BP376217STT
CSB-MP376217STT
CSB-EP376217STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Homoserine O-succinyltransferase (metA) CSB-YP376218STT
CSB-EP376218STT
CSB-BP376218STT
CSB-MP376218STT
CSB-EP376218STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Lipid-A-disaccharide synthase (lpxB) CSB-YP376219STT
CSB-EP376219STT
CSB-BP376219STT
CSB-MP376219STT
CSB-EP376219STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Uridylate kinase (pyrH) CSB-YP376220STT
CSB-EP376220STT
CSB-BP376220STT
CSB-MP376220STT
CSB-EP376220STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP376221STT
CSB-EP376221STT
CSB-BP376221STT
CSB-MP376221STT
CSB-EP376221STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. tRNA sulfurtransferase (thiI) CSB-YP376222STT
CSB-EP376222STT
CSB-BP376222STT
CSB-MP376222STT
CSB-EP376222STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP376223STT
CSB-EP376223STT
CSB-BP376223STT
CSB-MP376223STT
CSB-EP376223STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0597 protein Sputw3181_2955 (Sputw3181_2955) CSB-YP376224STT
CSB-EP376224STT
CSB-BP376224STT
CSB-MP376224STT
CSB-EP376224STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Hydroxylamine reductase (hcp), partial CSB-YP376225STT
CSB-EP376225STT
CSB-BP376225STT
CSB-MP376225STT
CSB-EP376225STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 30S ribosomal protein S16 (rpsP) CSB-YP376226STT
CSB-EP376226STT
CSB-BP376226STT
CSB-MP376226STT
CSB-EP376226STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Na (+)/H (+) antiporter nhaA, partial CSB-YP376227STT
CSB-EP376227STT
CSB-BP376227STT
CSB-MP376227STT
CSB-EP376227STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. RNA pyrophosphohydrolase (rppH) CSB-YP376228STT
CSB-EP376228STT
CSB-BP376228STT
CSB-MP376228STT
CSB-EP376228STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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