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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella sp. ATP-dependent RNA helicase rhlB (rhlB) CSB-YP376169STT
CSB-EP376169STT
CSB-BP376169STT
CSB-MP376169STT
CSB-EP376169STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. DNA gyrase inhibitor YacG (yacG) CSB-YP376170STT
CSB-EP376170STT
CSB-BP376170STT
CSB-MP376170STT
CSB-EP376170STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 3-dehydroquinate dehydratase (aroQ) CSB-YP376171STT
CSB-EP376171STT
CSB-BP376171STT
CSB-MP376171STT
CSB-EP376171STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP376172STT1
CSB-EP376172STT1
CSB-BP376172STT1
CSB-MP376172STT1
CSB-EP376172STT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Sugar fermentation stimulation protein homolog (sfsA) CSB-YP376173STT
CSB-EP376173STT
CSB-BP376173STT
CSB-MP376173STT
CSB-EP376173STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP376174STT
CSB-EP376174STT
CSB-BP376174STT
CSB-MP376174STT
CSB-EP376174STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Protein slyX homolog (slyX) CSB-YP376175STT
CSB-EP376175STT
CSB-BP376175STT
CSB-MP376175STT
CSB-EP376175STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Chaperone protein DnaK (dnaK), partial CSB-YP376176STT
CSB-EP376176STT
CSB-BP376176STT
CSB-MP376176STT
CSB-EP376176STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Cobalamin synthase (cobS), partial CSB-YP376177STT1
CSB-EP376177STT1
CSB-BP376177STT1
CSB-MP376177STT1
CSB-EP376177STT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP376178STT
CSB-EP376178STT
CSB-BP376178STT
CSB-MP376178STT
CSB-EP376178STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP376179STT
CSB-EP376179STT
CSB-BP376179STT
CSB-MP376179STT
CSB-EP376179STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 30S ribosomal protein S15 (rpsO) CSB-YP376180STT
CSB-EP376180STT
CSB-BP376180STT
CSB-MP376180STT
CSB-EP376180STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Transcriptional repressor NrdR (nrdR) CSB-YP376181STT
CSB-EP376181STT
CSB-BP376181STT
CSB-MP376181STT
CSB-EP376181STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Cell division protein FtsB (ftsB), partial CSB-YP376182STT
CSB-EP376182STT
CSB-BP376182STT
CSB-MP376182STT
CSB-EP376182STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP376183STT
CSB-EP376183STT
CSB-BP376183STT
CSB-MP376183STT
CSB-EP376183STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Carbon storage regulator homolog (csrA) CSB-YP376184STT
CSB-EP376184STT
CSB-BP376184STT
CSB-MP376184STT
CSB-EP376184STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP376185STT
CSB-EP376185STT
CSB-BP376185STT
CSB-MP376185STT
CSB-EP376185STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Serine/threonine transporter sstT (sstT), partial CSB-YP376186STT
CSB-EP376186STT
CSB-BP376186STT
CSB-MP376186STT
CSB-EP376186STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0115 protein Sputw3181_1555 (Sputw3181_1555) CSB-YP376187STT
CSB-EP376187STT
CSB-BP376187STT
CSB-MP376187STT
CSB-EP376187STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Tryptophan synthase beta chain (trpB) CSB-YP376188STT
CSB-EP376188STT
CSB-BP376188STT
CSB-MP376188STT
CSB-EP376188STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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