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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP376409STK
CSB-EP376409STK
CSB-BP376409STK
CSB-MP376409STK
CSB-EP376409STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Aspartate--tRNA ligase (aspS), partial CSB-YP376410STK
CSB-EP376410STK
CSB-BP376410STK
CSB-MP376410STK
CSB-EP376410STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP376411STK
CSB-EP376411STK
CSB-BP376411STK
CSB-MP376411STK
CSB-EP376411STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis D-alanine--D-alanine ligase (ddl) CSB-YP376412STK
CSB-EP376412STK
CSB-BP376412STK
CSB-MP376412STK
CSB-EP376412STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Disulfide bond formation protein B (dsbB), partial CSB-YP376413STK1
CSB-EP376413STK1
CSB-BP376413STK1
CSB-MP376413STK1
CSB-EP376413STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Cell division topological specificity factor (minE) CSB-YP376414STK
CSB-EP376414STK
CSB-BP376414STK
CSB-MP376414STK
CSB-EP376414STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP376415STK
CSB-EP376415STK
CSB-BP376415STK
CSB-MP376415STK
CSB-EP376415STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Integration host factor subunit alpha (ihfA) CSB-YP376416STK
CSB-EP376416STK
CSB-BP376416STK
CSB-MP376416STK
CSB-EP376416STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Biosynthetic arginine decarboxylase (speA), partial CSB-YP376417STK
CSB-EP376417STK
CSB-BP376417STK
CSB-MP376417STK
CSB-EP376417STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phosphate acyltransferase (plsX) CSB-YP376418STK
CSB-EP376418STK
CSB-BP376418STK
CSB-MP376418STK
CSB-EP376418STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Deoxycytidine triphosphate deaminase (dcd) CSB-YP376419STK
CSB-EP376419STK
CSB-BP376419STK
CSB-MP376419STK
CSB-EP376419STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Translation initiation factor IF-1 (infA) CSB-YP376420STK
CSB-EP376420STK
CSB-BP376420STK
CSB-MP376420STK
CSB-EP376420STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP376421STK
CSB-EP376421STK
CSB-BP376421STK
CSB-MP376421STK
CSB-EP376421STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Uracil phosphoribosyltransferase (upp) CSB-YP376422STK
CSB-EP376422STK
CSB-BP376422STK
CSB-MP376422STK
CSB-EP376422STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Tryptophan synthase beta chain (trpB) CSB-YP376423STK
CSB-EP376423STK
CSB-BP376423STK
CSB-MP376423STK
CSB-EP376423STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP376424STK
CSB-EP376424STK
CSB-BP376424STK
CSB-MP376424STK
CSB-EP376424STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP376425STK
CSB-EP376425STK
CSB-BP376425STK
CSB-MP376425STK
CSB-EP376425STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glutamate--tRNA ligase (gltX) CSB-YP376426STK
CSB-EP376426STK
CSB-BP376426STK
CSB-MP376426STK
CSB-EP376426STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Putative pterin-4-alpha-carbinolamine dehydratase (Sama_2221) CSB-YP376427STK
CSB-EP376427STK
CSB-BP376427STK
CSB-MP376427STK
CSB-EP376427STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Flagellar P-ring protein (flgI) CSB-YP376428STK
CSB-EP376428STK
CSB-BP376428STK
CSB-MP376428STK
CSB-EP376428STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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