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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum islandicum N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP376969PZW
CSB-EP376969PZW
CSB-BP376969PZW
CSB-MP376969PZW
CSB-EP376969PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Riboflavin kinase (ribK) CSB-YP376970TKC
CSB-EP376970TKC
CSB-BP376970TKC
CSB-MP376970TKC
CSB-EP376970TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 30S ribosomal protein S19P (rps19p) CSB-YP376971TKC
CSB-EP376971TKC
CSB-BP376971TKC
CSB-MP376971TKC
CSB-EP376971TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 50S ribosomal protein L18P (rpl18p) CSB-YP376972TKC
CSB-EP376972TKC
CSB-BP376972TKC
CSB-MP376972TKC
CSB-EP376972TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 30S ribosomal protein S4P (rps4p) CSB-YP376973TKC
CSB-EP376973TKC
CSB-BP376973TKC
CSB-MP376973TKC
CSB-EP376973TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Replication factor C small subunit (rfcS) CSB-YP376974TKC
CSB-EP376974TKC
CSB-BP376974TKC
CSB-MP376974TKC
CSB-EP376974TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 30S ribosomal protein S12P (rps12p) CSB-YP376975TKC
CSB-EP376975TKC
CSB-BP376975TKC
CSB-MP376975TKC
CSB-EP376975TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 50S ribosomal protein L40e (rpl40e) CSB-YP376976TKC
CSB-EP376976TKC
CSB-BP376976TKC
CSB-MP376976TKC
CSB-EP376976TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Proteasome subunit beta 1 (psmB1) CSB-YP376977TKC
CSB-EP376977TKC
CSB-BP376977TKC
CSB-MP376977TKC
CSB-EP376977TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens tRNA pseudouridine synthase Pus10 (pus10) CSB-YP376978TKC
CSB-EP376978TKC
CSB-BP376978TKC
CSB-MP376978TKC
CSB-EP376978TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP376979TKC
CSB-EP376979TKC
CSB-BP376979TKC
CSB-MP376979TKC
CSB-EP376979TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Translation initiation factor 5A (eIF5A) CSB-YP376980TKC
CSB-EP376980TKC
CSB-BP376980TKC
CSB-MP376980TKC
CSB-EP376980TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Prefoldin subunit alpha (pfdA) CSB-YP376981TKC
CSB-EP376981TKC
CSB-BP376981TKC
CSB-MP376981TKC
CSB-EP376981TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 50S ribosomal protein L14e (rpl14e) CSB-YP376982TKC
CSB-EP376982TKC
CSB-BP376982TKC
CSB-MP376982TKC
CSB-EP376982TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 50S ribosomal protein L14P (rpl14p) CSB-YP376983TKC
CSB-EP376983TKC
CSB-BP376983TKC
CSB-MP376983TKC
CSB-EP376983TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens 30S ribosomal protein S28e (rps28e) CSB-YP376984TKC
CSB-EP376984TKC
CSB-BP376984TKC
CSB-MP376984TKC
CSB-EP376984TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens UPF0095 protein Tpen_0501 (Tpen_0501) CSB-YP376985TKC
CSB-EP376985TKC
CSB-BP376985TKC
CSB-MP376985TKC
CSB-EP376985TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens RNA-binding protein AU-1 (aubA) CSB-YP376986TKC
CSB-EP376986TKC
CSB-BP376986TKC
CSB-MP376986TKC
CSB-EP376986TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Probable exosome complex RNA-binding protein 1 (Tpen_0582) CSB-YP376987TKC
CSB-EP376987TKC
CSB-BP376987TKC
CSB-MP376987TKC
CSB-EP376987TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Methionine--tRNA ligase (metG), partial CSB-YP376988TKC
CSB-EP376988TKC
CSB-BP376988TKC
CSB-MP376988TKC
CSB-EP376988TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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