Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermofilum pendens Aspartate carbamoyltransferase (pyrB) CSB-YP377649TKC
CSB-EP377649TKC
CSB-BP377649TKC
CSB-MP377649TKC
CSB-EP377649TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP377650TKC
CSB-EP377650TKC
CSB-BP377650TKC
CSB-MP377650TKC
CSB-EP377650TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens UPF0173 metal-dependent hydrolase Tpen_1493 (Tpen_1493) CSB-YP377651TKC
CSB-EP377651TKC
CSB-BP377651TKC
CSB-MP377651TKC
CSB-EP377651TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA modification GTPase MnmE (mnmE) CSB-YP377652STK
CSB-EP377652STK
CSB-BP377652STK
CSB-MP377652STK
CSB-EP377652STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribonuclease P protein component (rnpA) CSB-YP377653STK
CSB-EP377653STK
CSB-BP377653STK
CSB-MP377653STK
CSB-EP377653STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA replication and repair protein recF (recF) CSB-YP377654STK
CSB-EP377654STK
CSB-BP377654STK
CSB-MP377654STK
CSB-EP377654STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP377655STK
CSB-EP377655STK
CSB-BP377655STK
CSB-MP377655STK
CSB-EP377655STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP377656STK
CSB-EP377656STK
CSB-BP377656STK
CSB-MP377656STK
CSB-EP377656STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP377657STK
CSB-EP377657STK
CSB-BP377657STK
CSB-MP377657STK
CSB-EP377657STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP377658STK
CSB-EP377658STK
CSB-BP377658STK
CSB-MP377658STK
CSB-EP377658STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP377659STK
CSB-EP377659STK
CSB-BP377659STK
CSB-MP377659STK
CSB-EP377659STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA (uracil (54)-C (5))-methyltransferase CSB-YP377660STK
CSB-EP377660STK
CSB-BP377660STK
CSB-MP377660STK
CSB-EP377660STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L1 (rplA) CSB-YP377661STK
CSB-EP377661STK
CSB-BP377661STK
CSB-MP377661STK
CSB-EP377661STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP377662STK
CSB-EP377662STK
CSB-BP377662STK
CSB-MP377662STK
CSB-EP377662STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S10 (rpsJ) CSB-YP377663STK
CSB-EP377663STK
CSB-BP377663STK
CSB-MP377663STK
CSB-EP377663STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L2 (rplB) CSB-YP377664STK
CSB-EP377664STK
CSB-BP377664STK
CSB-MP377664STK
CSB-EP377664STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S19 (rpsS) CSB-YP377665STK
CSB-EP377665STK
CSB-BP377665STK
CSB-MP377665STK
CSB-EP377665STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L16 (rplP) CSB-YP377666STK
CSB-EP377666STK
CSB-BP377666STK
CSB-MP377666STK
CSB-EP377666STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S17 (rpsQ) CSB-YP377667STK
CSB-EP377667STK
CSB-BP377667STK
CSB-MP377667STK
CSB-EP377667STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L24 (rplX) CSB-YP377668STK
CSB-EP377668STK
CSB-BP377668STK
CSB-MP377668STK
CSB-EP377668STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>