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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Halorhodospira halophila Malonyl-CoA O-methyltransferase BioC (bioC) CSB-YP379630HSG
CSB-EP379630HSG
CSB-BP379630HSG
CSB-MP379630HSG
CSB-EP379630HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP379631HSG
CSB-EP379631HSG
CSB-BP379631HSG
CSB-MP379631HSG
CSB-EP379631HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 50S ribosomal protein L25 (rplY) CSB-YP379632HSG
CSB-EP379632HSG
CSB-BP379632HSG
CSB-MP379632HSG
CSB-EP379632HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 50S ribosomal protein L25 (rplY) CSB-YP379633BOR
CSB-EP379633BOR
CSB-BP379633BOR
CSB-MP379633BOR
CSB-EP379633BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP379634BOR
CSB-EP379634BOR
CSB-BP379634BOR
CSB-MP379634BOR
CSB-EP379634BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP379635BOR
CSB-EP379635BOR
CSB-BP379635BOR
CSB-MP379635BOR
CSB-EP379635BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis UPF0133 protein BARBAKC583_1239 (BARBAKC583_1239) CSB-YP379636BOR
CSB-EP379636BOR
CSB-BP379636BOR
CSB-MP379636BOR
CSB-EP379636BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Recombination protein RecR (recR) CSB-YP379637BOR
CSB-EP379637BOR
CSB-BP379637BOR
CSB-MP379637BOR
CSB-EP379637BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis tRNA (guanine-N (7)-)-methyltransferase CSB-YP379638BOR
CSB-EP379638BOR
CSB-BP379638BOR
CSB-MP379638BOR
CSB-EP379638BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP379639BOR
CSB-EP379639BOR
CSB-BP379639BOR
CSB-MP379639BOR
CSB-EP379639BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Protein-export protein SecB (secB) CSB-YP379640BOR
CSB-EP379640BOR
CSB-BP379640BOR
CSB-MP379640BOR
CSB-EP379640BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 50S ribosomal protein L20 (rplT) CSB-YP379641BOR
CSB-EP379641BOR
CSB-BP379641BOR
CSB-MP379641BOR
CSB-EP379641BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 50S ribosomal protein L35 (rpmI) CSB-YP379642BOR
CSB-EP379642BOR
CSB-BP379642BOR
CSB-MP379642BOR
CSB-EP379642BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP379643BOR
CSB-EP379643BOR
CSB-BP379643BOR
CSB-MP379643BOR
CSB-EP379643BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP379644BOR
CSB-EP379644BOR
CSB-BP379644BOR
CSB-MP379644BOR
CSB-EP379644BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Betaine aldehyde dehydrogenase (betB) CSB-YP379645BPR
CSB-EP379645BPR
CSB-BP379645BPR
CSB-MP379645BPR
CSB-EP379645BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Indole-3-glycerol phosphate synthase (trpC) CSB-YP379646BPR
CSB-EP379646BPR
CSB-BP379646BPR
CSB-MP379646BPR
CSB-EP379646BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Homoserine kinase (thrB) CSB-YP379647BPR
CSB-EP379647BPR
CSB-BP379647BPR
CSB-MP379647BPR
CSB-EP379647BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei GTP cyclohydrolase folE2 (folE2) CSB-YP379648BPR
CSB-EP379648BPR
CSB-BP379648BPR
CSB-MP379648BPR
CSB-EP379648BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP379649BPR
CSB-EP379649BPR
CSB-BP379649BPR
CSB-MP379649BPR
CSB-EP379649BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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