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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Verminephrobacter eiseniae Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP379590VDX
CSB-EP379590VDX
CSB-BP379590VDX
CSB-MP379590VDX
CSB-EP379590VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Non-canonical purine NTP pyrophosphatase (Veis_4156) CSB-YP379591VDX
CSB-EP379591VDX
CSB-BP379591VDX
CSB-MP379591VDX
CSB-EP379591VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Uracil phosphoribosyltransferase (upp) CSB-YP379592VDX
CSB-EP379592VDX
CSB-BP379592VDX
CSB-MP379592VDX
CSB-EP379592VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Transcriptional repressor NrdR (nrdR) CSB-YP379593VDX
CSB-EP379593VDX
CSB-BP379593VDX
CSB-MP379593VDX
CSB-EP379593VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Trigger factor (tig) CSB-YP379594VDX
CSB-EP379594VDX
CSB-BP379594VDX
CSB-MP379594VDX
CSB-EP379594VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP379595VDX
CSB-EP379595VDX
CSB-BP379595VDX
CSB-MP379595VDX
CSB-EP379595VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae DNA ligase (ligA), partial CSB-YP379596VDX
CSB-EP379596VDX
CSB-BP379596VDX
CSB-MP379596VDX
CSB-EP379596VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae DNA mismatch repair protein MutS (mutS), partial CSB-YP379597VDX
CSB-EP379597VDX
CSB-BP379597VDX
CSB-MP379597VDX
CSB-EP379597VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Protein MraZ (mraZ) CSB-YP379598VDX
CSB-EP379598VDX
CSB-BP379598VDX
CSB-MP379598VDX
CSB-EP379598VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae UPF0317 protein Veis_4744 (Veis_4744) CSB-YP379599VDX
CSB-EP379599VDX
CSB-BP379599VDX
CSB-MP379599VDX
CSB-EP379599VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP379600VDX
CSB-EP379600VDX
CSB-BP379600VDX
CSB-MP379600VDX
CSB-EP379600VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae tRNA modification GTPase MnmE (mnmE) CSB-YP379601VDX
CSB-EP379601VDX
CSB-BP379601VDX
CSB-MP379601VDX
CSB-EP379601VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP379602HSG
CSB-EP379602HSG
CSB-BP379602HSG
CSB-MP379602HSG
CSB-EP379602HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP379603HSG
CSB-EP379603HSG
CSB-BP379603HSG
CSB-MP379603HSG
CSB-EP379603HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP379604HSG
CSB-EP379604HSG
CSB-BP379604HSG
CSB-MP379604HSG
CSB-EP379604HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Ribosomal protein S6 modification protein (rimK) CSB-YP379605HSG
CSB-EP379605HSG
CSB-BP379605HSG
CSB-MP379605HSG
CSB-EP379605HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 50S ribosomal protein L35 (rpmI) CSB-YP379606HSG
CSB-EP379606HSG
CSB-BP379606HSG
CSB-MP379606HSG
CSB-EP379606HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 50S ribosomal protein L19 (rplS) CSB-YP379607HSG
CSB-EP379607HSG
CSB-BP379607HSG
CSB-MP379607HSG
CSB-EP379607HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Catalase-peroxidase (katG), partial CSB-YP379608HSG
CSB-EP379608HSG
CSB-BP379608HSG
CSB-MP379608HSG
CSB-EP379608HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP379609HSG
CSB-EP379609HSG
CSB-BP379609HSG
CSB-MP379609HSG
CSB-EP379609HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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