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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Halorhodospira halophila Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP380590HSG
CSB-EP380590HSG
CSB-BP380590HSG
CSB-MP380590HSG
CSB-EP380590HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Phosphopantetheine adenylyltransferase (coaD) CSB-YP380591HSG
CSB-EP380591HSG
CSB-BP380591HSG
CSB-MP380591HSG
CSB-EP380591HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 50S ribosomal protein L28 (rpmB) CSB-YP380592HSG
CSB-EP380592HSG
CSB-BP380592HSG
CSB-MP380592HSG
CSB-EP380592HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP380593HSG
CSB-EP380593HSG
CSB-BP380593HSG
CSB-MP380593HSG
CSB-EP380593HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Protein smg homolog (smg) CSB-YP380594HSG
CSB-EP380594HSG
CSB-BP380594HSG
CSB-MP380594HSG
CSB-EP380594HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP380595HSG
CSB-EP380595HSG
CSB-BP380595HSG
CSB-MP380595HSG
CSB-EP380595HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Multifunctional CCA protein (cca) CSB-YP380596HSG
CSB-EP380596HSG
CSB-BP380596HSG
CSB-MP380596HSG
CSB-EP380596HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 60 kDa chaperonin (groL), partial CSB-YP380597HSG
CSB-EP380597HSG
CSB-BP380597HSG
CSB-MP380597HSG
CSB-EP380597HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila tRNA (guanine-N (7)-)-methyltransferase CSB-YP380598HSG
CSB-EP380598HSG
CSB-BP380598HSG
CSB-MP380598HSG
CSB-EP380598HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Arginine--tRNA ligase (argS), partial CSB-YP380599HSG
CSB-EP380599HSG
CSB-BP380599HSG
CSB-MP380599HSG
CSB-EP380599HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Bifunctional protein GlmU (glmU) CSB-YP380600HSG
CSB-EP380600HSG
CSB-BP380600HSG
CSB-MP380600HSG
CSB-EP380600HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila ATP synthase gamma chain (atpG) CSB-YP380601HSG
CSB-EP380601HSG
CSB-BP380601HSG
CSB-MP380601HSG
CSB-EP380601HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echinops telfairi Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 (ASZ1) CSB-YP380602EJK
CSB-EP380602EJK
CSB-BP380602EJK
CSB-MP380602EJK
CSB-EP380602EJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perna viridis Foot protein 1 variant 2 (fp-1) CSB-YP380603PYH
CSB-EP380603PYH
CSB-BP380603PYH
CSB-MP380603PYH
CSB-EP380603PYH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Peptidyl-tRNA hydrolase (pth) CSB-YP380604BOR
CSB-EP380604BOR
CSB-BP380604BOR
CSB-MP380604BOR
CSB-EP380604BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Probable GTP-binding protein EngB (engB) CSB-YP380605BOR
CSB-EP380605BOR
CSB-BP380605BOR
CSB-MP380605BOR
CSB-EP380605BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis UPF0434 protein BARBAKC583_1098 (BARBAKC583_1098) CSB-YP380606BOR
CSB-EP380606BOR
CSB-BP380606BOR
CSB-MP380606BOR
CSB-EP380606BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 10 kDa chaperonin (groS) CSB-YP380607BOR
CSB-EP380607BOR
CSB-BP380607BOR
CSB-MP380607BOR
CSB-EP380607BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP380608BOR
CSB-EP380608BOR
CSB-BP380608BOR
CSB-MP380608BOR
CSB-EP380608BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP380609BOR
CSB-EP380609BOR
CSB-BP380609BOR
CSB-MP380609BOR
CSB-EP380609BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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