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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Verminephrobacter eiseniae 60 kDa chaperonin (groL), partial CSB-YP380990VDX
CSB-EP380990VDX
CSB-BP380990VDX
CSB-MP380990VDX
CSB-EP380990VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Chorismate synthase (aroC) CSB-YP380991VDX
CSB-EP380991VDX
CSB-BP380991VDX
CSB-MP380991VDX
CSB-EP380991VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Enolase (eno) CSB-YP380992VDX
CSB-EP380992VDX
CSB-BP380992VDX
CSB-MP380992VDX
CSB-EP380992VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae tRNA pseudouridine synthase B (truB) CSB-YP380993VDX
CSB-EP380993VDX
CSB-BP380993VDX
CSB-MP380993VDX
CSB-EP380993VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP380994VDX
CSB-EP380994VDX
CSB-BP380994VDX
CSB-MP380994VDX
CSB-EP380994VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP380995VDX
CSB-EP380995VDX
CSB-BP380995VDX
CSB-MP380995VDX
CSB-EP380995VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP380996VDX
CSB-EP380996VDX
CSB-BP380996VDX
CSB-MP380996VDX
CSB-EP380996VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L20 (rplT) CSB-YP380997VDX
CSB-EP380997VDX
CSB-BP380997VDX
CSB-MP380997VDX
CSB-EP380997VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP380998VDX
CSB-EP380998VDX
CSB-BP380998VDX
CSB-MP380998VDX
CSB-EP380998VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP380999VDX
CSB-EP380999VDX
CSB-BP380999VDX
CSB-MP380999VDX
CSB-EP380999VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Imidazolonepropionase (hutI) CSB-YP381000VDX
CSB-EP381000VDX
CSB-BP381000VDX
CSB-MP381000VDX
CSB-EP381000VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae SsrA-binding protein (smpB) CSB-YP381001VDX
CSB-EP381001VDX
CSB-BP381001VDX
CSB-MP381001VDX
CSB-EP381001VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L21 (rplU) CSB-YP381002VDX
CSB-EP381002VDX
CSB-BP381002VDX
CSB-MP381002VDX
CSB-EP381002VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Probable rRNA maturation factor (Veis_4066) CSB-YP381003VDX
CSB-EP381003VDX
CSB-BP381003VDX
CSB-MP381003VDX
CSB-EP381003VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP381004VDX
CSB-EP381004VDX
CSB-BP381004VDX
CSB-MP381004VDX
CSB-EP381004VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Probable transcriptional regulatory protein Veis_4238 (Veis_4238) CSB-YP381005VDX
CSB-EP381005VDX
CSB-BP381005VDX
CSB-MP381005VDX
CSB-EP381005VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Alanine racemase (alr) CSB-YP381006VDX
CSB-EP381006VDX
CSB-BP381006VDX
CSB-MP381006VDX
CSB-EP381006VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP381007VDX
CSB-EP381007VDX
CSB-BP381007VDX
CSB-MP381007VDX
CSB-EP381007VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP381008VDX
CSB-EP381008VDX
CSB-BP381008VDX
CSB-MP381008VDX
CSB-EP381008VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP381009VDX
CSB-EP381009VDX
CSB-BP381009VDX
CSB-MP381009VDX
CSB-EP381009VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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