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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidovorax sp. Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP380910AUC1
CSB-EP380910AUC1
CSB-BP380910AUC1
CSB-MP380910AUC1
CSB-EP380910AUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP380911AUC
CSB-EP380911AUC
CSB-BP380911AUC
CSB-MP380911AUC
CSB-EP380911AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP380912AUC
CSB-EP380912AUC
CSB-BP380912AUC
CSB-MP380912AUC
CSB-EP380912AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP380913AUC
CSB-EP380913AUC
CSB-BP380913AUC
CSB-MP380913AUC
CSB-EP380913AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP380914AUC
CSB-EP380914AUC
CSB-BP380914AUC
CSB-MP380914AUC
CSB-EP380914AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP380915AUC
CSB-EP380915AUC
CSB-BP380915AUC
CSB-MP380915AUC
CSB-EP380915AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Adenylosuccinate synthetase (purA) CSB-YP380916AUC
CSB-EP380916AUC
CSB-BP380916AUC
CSB-MP380916AUC
CSB-EP380916AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP380917AUC
CSB-EP380917AUC
CSB-BP380917AUC
CSB-MP380917AUC
CSB-EP380917AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Putative thymidine phosphorylase 1 (Ajs_1507), partial CSB-YP380918AUC
CSB-EP380918AUC
CSB-BP380918AUC
CSB-MP380918AUC
CSB-EP380918AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Chorismate synthase (aroC) CSB-YP380919AUC
CSB-EP380919AUC
CSB-BP380919AUC
CSB-MP380919AUC
CSB-EP380919AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 2-isopropylmalate synthase (leuA), partial CSB-YP380920AUC
CSB-EP380920AUC
CSB-BP380920AUC
CSB-MP380920AUC
CSB-EP380920AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Alanine--tRNA ligase (alaS), partial CSB-YP380921AUC
CSB-EP380921AUC
CSB-BP380921AUC
CSB-MP380921AUC
CSB-EP380921AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Biotin synthase (bioB) CSB-YP380922AUC
CSB-EP380922AUC
CSB-BP380922AUC
CSB-MP380922AUC
CSB-EP380922AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP380923AUC
CSB-EP380923AUC
CSB-BP380923AUC
CSB-MP380923AUC
CSB-EP380923AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. DNA ligase (ligA), partial CSB-YP380924AUC
CSB-EP380924AUC
CSB-BP380924AUC
CSB-MP380924AUC
CSB-EP380924AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. UPF0434 protein Ajs_2306 (Ajs_2306) CSB-YP380925AUC
CSB-EP380925AUC
CSB-BP380925AUC
CSB-MP380925AUC
CSB-EP380925AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP380926AUC
CSB-EP380926AUC
CSB-BP380926AUC
CSB-MP380926AUC
CSB-EP380926AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Disulfide bond formation protein B (dsbB), partial CSB-YP380927AUC1
CSB-EP380927AUC1
CSB-BP380927AUC1
CSB-MP380927AUC1
CSB-EP380927AUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. FMN-dependent NADH-azoreductase (azoR) CSB-YP380928AUC
CSB-EP380928AUC
CSB-BP380928AUC
CSB-MP380928AUC
CSB-EP380928AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP380929AUC
CSB-EP380929AUC
CSB-BP380929AUC
CSB-MP380929AUC
CSB-EP380929AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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