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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Cytosolic phospholipase A2 beta (Pla2g4b) CSB-YP018098MO
CSB-EP018098MO
CSB-BP018098MO
CSB-MP018098MO
CSB-EP018098MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse JmjC domain-containing protein 7 (Jmjd7) CSB-YP011955MO
CSB-EP011955MO
CSB-BP011955MO
CSB-MP011955MO
CSB-EP011955MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein-tyrosine phosphatase mitochondrial 1 (Ptpmt1) CSB-YP019023RA
CSB-EP019023RA
CSB-BP019023RA
CSB-MP019023RA
CSB-EP019023RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Hepatitis A virus cellular receptor 2 homolog (Havcr2), partial CSB-YP010145RA
CSB-EP010145RA
CSB-BP010145RA
CSB-MP010145RA
CSB-EP010145RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Tuberoinfundibular peptide of 39 residues (PTH2) CSB-YP018989BO
CSB-EP018989BO
CSB-BP018989BO
CSB-MP018989BO
CSB-EP018989BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat AP2-associated protein kinase 1 (Aak1), partial CSB-YP001020RA
CSB-EP001020RA
CSB-BP001020RA
CSB-MP001020RA
CSB-EP001020RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human TCF3 fusion partner (TFPT) CSB-YP023439HU
CSB-EP023439HU
CSB-BP023439HU
CSB-MP023439HU
CSB-EP023439HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C14orf38 (C14orf38), partial CSB-YP003192HU
CSB-EP003192HU
CSB-BP003192HU
CSB-MP003192HU
CSB-EP003192HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 39S ribosomal protein L55, mitochondrial (MRPL55) CSB-YP014872BO
CSB-EP014872BO
CSB-BP014872BO
CSB-MP014872BO
CSB-EP014872BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cortexin-2 (CTXN2), partial CSB-YP006211HU1
CSB-EP006211HU1
CSB-BP006211HU1
CSB-MP006211HU1
CSB-EP006211HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Charged multivesicular body protein 6 (Chmp6) CSB-YP005366MO
CSB-EP005366MO
CSB-BP005366MO
CSB-MP005366MO
CSB-EP005366MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ras-related protein Rab-40A-like (RAB40AL) CSB-YP019203HU
CSB-EP019203HU
CSB-BP019203HU
CSB-MP019203HU
CSB-EP019203HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone H2A type 1 (HIST1H2AG) CSB-YP010389HU
CSB-BP010389HU
CSB-MP010389HU
CSB-EP010389HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Probable G-protein coupled receptor 139 (Gpr139), partial CSB-YP009755RA1
CSB-EP009755RA1
CSB-BP009755RA1
CSB-MP009755RA1
CSB-EP009755RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tuberoinfundibular peptide of 39 residues (Pth2) CSB-YP018989RA
CSB-EP018989RA
CSB-BP018989RA
CSB-MP018989RA
CSB-EP018989RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y (Pigy), partial CSB-YP017988MO1
CSB-EP017988MO1
CSB-BP017988MO1
CSB-MP017988MO1
CSB-EP017988MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 39S ribosomal protein L52, mitochondrial (MRPL52) CSB-YP014869BO
CSB-EP014869BO
CSB-BP014869BO
CSB-MP014869BO
CSB-EP014869BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Phosphoinositide 3-kinase regulatory subunit 4 (Pik3r4), partial CSB-YP018007RA
CSB-EP018007RA
CSB-BP018007RA
CSB-MP018007RA
CSB-EP018007RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone H2B type F-M (H2BFM) CSB-YP010103HU
CSB-EP010103HU
CSB-BP010103HU
CSB-MP010103HU
CSB-EP010103HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Ubiquitin-60S ribosomal protein L40 (UBA52) CSB-YP025416SH
CSB-EP025416SH
CSB-BP025416SH
CSB-MP025416SH
CSB-EP025416SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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