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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse MAX gene-associated protein (Mga), partial CSB-YP381793MO
CSB-EP381793MO
CSB-BP381793MO
CSB-MP381793MO
CSB-EP381793MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase Cfr (cfr) CSB-YP381794SUO
CSB-EP381794SUO
CSB-BP381794SUO
CSB-MP381794SUO
CSB-EP381794SUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transmembrane protein 11-A, mitochondrial (tmem11-a), partial CSB-YP381795XBE1
CSB-EP381795XBE1
CSB-BP381795XBE1
CSB-MP381795XBE1
CSB-EP381795XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Actin, cytoplasmic 2 (actg1) CSB-YP381796XBE
CSB-EP381796XBE
CSB-BP381796XBE
CSB-MP381796XBE
CSB-EP381796XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ventricular zone-expressed PH domain-containing protein (veph), partial CSB-YP381797DIL
CSB-EP381797DIL
CSB-BP381797DIL
CSB-MP381797DIL
CSB-EP381797DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio B-cell CLL/lymphoma 7 protein family member B-B (bcl7bb) CSB-YP381798DIL
CSB-EP381798DIL
CSB-BP381798DIL
CSB-MP381798DIL
CSB-EP381798DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus tRNA (Ile2) 2-agmatinylcytidine synthetase TiaS CSB-YP381799HSR
CSB-EP381799HSR
CSB-BP381799HSR
CSB-MP381799HSR
CSB-EP381799HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Protein translation factor SUI1 homolog (Hbut_0053) CSB-YP381800HSR
CSB-EP381800HSR
CSB-BP381800HSR
CSB-MP381800HSR
CSB-EP381800HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Glutamyl-tRNA reductase (hemA) CSB-YP381801HSR
CSB-EP381801HSR
CSB-BP381801HSR
CSB-MP381801HSR
CSB-EP381801HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus 30S ribosomal protein S15P/S13e (rps15p) CSB-YP381802HSR
CSB-EP381802HSR
CSB-BP381802HSR
CSB-MP381802HSR
CSB-EP381802HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus UPF0218 protein Hbut_0437 (Hbut_0437) CSB-YP381803HSR
CSB-EP381803HSR
CSB-BP381803HSR
CSB-MP381803HSR
CSB-EP381803HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Probable translation initiation factor IF-2 (infB), partial CSB-YP381804HSR
CSB-EP381804HSR
CSB-BP381804HSR
CSB-MP381804HSR
CSB-EP381804HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus 30S ribosomal protein S13P (rps13p) CSB-YP381805HSR
CSB-EP381805HSR
CSB-BP381805HSR
CSB-MP381805HSR
CSB-EP381805HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus DNA-directed RNA polymerase subunit N (rpoN) CSB-YP381806HSR
CSB-EP381806HSR
CSB-BP381806HSR
CSB-MP381806HSR
CSB-EP381806HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus 50S ribosomal protein L7Ae (rpl7Ae) CSB-YP381807HSR
CSB-EP381807HSR
CSB-BP381807HSR
CSB-MP381807HSR
CSB-EP381807HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP381808HSR
CSB-EP381808HSR
CSB-BP381808HSR
CSB-MP381808HSR
CSB-EP381808HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Probable porphobilinogen deaminase (hemC) CSB-YP381809HSR
CSB-EP381809HSR
CSB-BP381809HSR
CSB-MP381809HSR
CSB-EP381809HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Bifunctional NAD (P)H-hydrate repair enzyme Nnr, partial CSB-YP381810HSR
CSB-EP381810HSR
CSB-BP381810HSR
CSB-MP381810HSR
CSB-EP381810HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP381811HSR
CSB-EP381811HSR
CSB-BP381811HSR
CSB-MP381811HSR
CSB-EP381811HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus 5'-nucleotidase surE (surE) CSB-YP381812HSR
CSB-EP381812HSR
CSB-BP381812HSR
CSB-MP381812HSR
CSB-EP381812HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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