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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus sanguinis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP384958SUR
CSB-EP384958SUR
CSB-BP384958SUR
CSB-MP384958SUR
CSB-EP384958SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Translation initiation factor IF-1 (infA) CSB-YP384959SUR
CSB-EP384959SUR
CSB-BP384959SUR
CSB-MP384959SUR
CSB-EP384959SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 50S ribosomal protein L36 (rpmJ) CSB-YP384960SUR
CSB-EP384960SUR
CSB-BP384960SUR
CSB-MP384960SUR
CSB-EP384960SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP384961SUR
CSB-EP384961SUR
CSB-BP384961SUR
CSB-MP384961SUR
CSB-EP384961SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis UPF0371 protein SSA_0208 (SSA_0208) CSB-YP384962SUR
CSB-EP384962SUR
CSB-BP384962SUR
CSB-MP384962SUR
CSB-EP384962SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Leucine--tRNA ligase (leuS), partial CSB-YP384963SUR
CSB-EP384963SUR
CSB-BP384963SUR
CSB-MP384963SUR
CSB-EP384963SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis UPF0356 protein SSA_0313 (SSA_0313) CSB-YP384964SUR
CSB-EP384964SUR
CSB-BP384964SUR
CSB-MP384964SUR
CSB-EP384964SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Xaa-Pro dipeptidyl-peptidase (pepX), partial CSB-YP384965SUR
CSB-EP384965SUR
CSB-BP384965SUR
CSB-MP384965SUR
CSB-EP384965SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Imidazolonepropionase (hutI) CSB-YP384966SUR
CSB-EP384966SUR
CSB-BP384966SUR
CSB-MP384966SUR
CSB-EP384966SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 30S ribosomal protein S6 (rpsF) CSB-YP384967SUR
CSB-EP384967SUR
CSB-BP384967SUR
CSB-MP384967SUR
CSB-EP384967SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Cobalamin biosynthesis protein CobD (cobD), partial CSB-YP384968SUR1
CSB-EP384968SUR1
CSB-BP384968SUR1
CSB-MP384968SUR1
CSB-EP384968SUR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Protein translocase subunit SecA 1 (secA1), partial CSB-YP384969SUR
CSB-EP384969SUR
CSB-BP384969SUR
CSB-MP384969SUR
CSB-EP384969SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP384970SUR
CSB-EP384970SUR
CSB-BP384970SUR
CSB-MP384970SUR
CSB-EP384970SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Protease HtpX homolog (htpX), partial CSB-YP384971SUR1
CSB-EP384971SUR1
CSB-BP384971SUR1
CSB-MP384971SUR1
CSB-EP384971SUR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP384972SUR
CSB-EP384972SUR
CSB-BP384972SUR
CSB-MP384972SUR
CSB-EP384972SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP384973SUR
CSB-EP384973SUR
CSB-BP384973SUR
CSB-MP384973SUR
CSB-EP384973SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Bifunctional protein FolD (folD) CSB-YP384974SUR
CSB-EP384974SUR
CSB-BP384974SUR
CSB-MP384974SUR
CSB-EP384974SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP384975SUR
CSB-EP384975SUR
CSB-BP384975SUR
CSB-MP384975SUR
CSB-EP384975SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis D-alanine--D-alanine ligase (ddl) CSB-YP384976SUR
CSB-EP384976SUR
CSB-BP384976SUR
CSB-MP384976SUR
CSB-EP384976SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Arginine deiminase (arcA) CSB-YP384977SUR
CSB-EP384977SUR
CSB-BP384977SUR
CSB-MP384977SUR
CSB-EP384977SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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