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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Outer-membrane lipoprotein lolB (lolB) CSB-YP385078STL
CSB-EP385078STL
CSB-BP385078STL
CSB-MP385078STL
CSB-EP385078STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Catalase-peroxidase (katG), partial CSB-YP385079STL
CSB-EP385079STL
CSB-BP385079STL
CSB-MP385079STL
CSB-EP385079STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA polymerase IV (dinB) CSB-YP385080STL
CSB-EP385080STL
CSB-BP385080STL
CSB-MP385080STL
CSB-EP385080STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GTPase obg (obg) CSB-YP385081STL
CSB-EP385081STL
CSB-BP385081STL
CSB-MP385081STL
CSB-EP385081STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP385082STL
CSB-EP385082STL
CSB-BP385082STL
CSB-MP385082STL
CSB-EP385082STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Isoleucine--tRNA ligase (ileS), partial CSB-YP385083STL
CSB-EP385083STL
CSB-BP385083STL
CSB-MP385083STL
CSB-EP385083STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP385084STL
CSB-EP385084STL
CSB-BP385084STL
CSB-MP385084STL
CSB-EP385084STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Na (+)/H (+) antiporter nhaA, partial CSB-YP385085STL
CSB-EP385085STL
CSB-BP385085STL
CSB-MP385085STL
CSB-EP385085STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Hydroxylamine reductase (hcp), partial CSB-YP385086STL
CSB-EP385086STL
CSB-BP385086STL
CSB-MP385086STL
CSB-EP385086STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP385087STL
CSB-EP385087STL
CSB-BP385087STL
CSB-MP385087STL
CSB-EP385087STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein grpE (grpE) CSB-YP385088STL
CSB-EP385088STL
CSB-BP385088STL
CSB-MP385088STL
CSB-EP385088STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA sulfurtransferase (thiI) CSB-YP385089STL
CSB-EP385089STL
CSB-BP385089STL
CSB-MP385089STL
CSB-EP385089STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein Xni (xni) CSB-YP385090STL
CSB-EP385090STL
CSB-BP385090STL
CSB-MP385090STL
CSB-EP385090STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S2 (rpsB) CSB-YP385091STL
CSB-EP385091STL
CSB-BP385091STL
CSB-MP385091STL
CSB-EP385091STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribonuclease HII (rnhB) CSB-YP385092STL
CSB-EP385092STL
CSB-BP385092STL
CSB-MP385092STL
CSB-EP385092STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cysteine--tRNA ligase (cysS) CSB-YP385093STL
CSB-EP385093STL
CSB-BP385093STL
CSB-MP385093STL
CSB-EP385093STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GTP cyclohydrolase-2 (ribA) CSB-YP385094STL
CSB-EP385094STL
CSB-BP385094STL
CSB-MP385094STL
CSB-EP385094STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphate acyltransferase (plsX) CSB-YP385095STL
CSB-EP385095STL
CSB-BP385095STL
CSB-MP385095STL
CSB-EP385095STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0178 protein Sbal_1771 (Sbal_1771) CSB-YP385096STL
CSB-EP385096STL
CSB-BP385096STL
CSB-MP385096STL
CSB-EP385096STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable septum site-determining protein MinC (minC) CSB-YP385097STL
CSB-EP385097STL
CSB-BP385097STL
CSB-MP385097STL
CSB-EP385097STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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