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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanoculleus marisnigri Aspartate--tRNA ligase (aspS) CSB-YP385058MNU
CSB-EP385058MNU
CSB-BP385058MNU
CSB-MP385058MNU
CSB-EP385058MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Translation initiation factor 2 subunit beta (eIF2B) CSB-YP385059MNU
CSB-EP385059MNU
CSB-BP385059MNU
CSB-MP385059MNU
CSB-EP385059MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 7-cyano-7-deazaguanine synthase (queC) CSB-YP385060MNU
CSB-EP385060MNU
CSB-BP385060MNU
CSB-MP385060MNU
CSB-EP385060MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 50S ribosomal protein L40e (rpl40e) CSB-YP385061MNU
CSB-EP385061MNU
CSB-BP385061MNU
CSB-MP385061MNU
CSB-EP385061MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Lysine--tRNA ligase (lysS), partial CSB-YP385062MNU
CSB-EP385062MNU
CSB-BP385062MNU
CSB-MP385062MNU
CSB-EP385062MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Elongation factor 1-beta (ef1b) CSB-YP385063MNU
CSB-EP385063MNU
CSB-BP385063MNU
CSB-MP385063MNU
CSB-EP385063MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chromosomal replication initiator protein DnaA (dnaA) CSB-YP385064STL
CSB-EP385064STL
CSB-BP385064STL
CSB-MP385064STL
CSB-EP385064STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Sulfurtransferase TusA homolog (tusA) CSB-YP385065STL
CSB-EP385065STL
CSB-BP385065STL
CSB-MP385065STL
CSB-EP385065STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Shikimate dehydrogenase (aroE) CSB-YP385066STL
CSB-EP385066STL
CSB-BP385066STL
CSB-MP385066STL
CSB-EP385066STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable GTP-binding protein EngB (engB) CSB-YP385067STL
CSB-EP385067STL
CSB-BP385067STL
CSB-MP385067STL
CSB-EP385067STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP385068STL
CSB-EP385068STL
CSB-BP385068STL
CSB-MP385068STL
CSB-EP385068STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP385069STL
CSB-EP385069STL
CSB-BP385069STL
CSB-MP385069STL
CSB-EP385069STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0761 membrane protein Sbal_0315 (Sbal_0315), partial CSB-YP385070STL1
CSB-EP385070STL1
CSB-BP385070STL1
CSB-MP385070STL1
CSB-EP385070STL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica FMN-dependent NADH-azoreductase (azoR) CSB-YP385071STL
CSB-EP385071STL
CSB-BP385071STL
CSB-MP385071STL
CSB-EP385071STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Nucleoid occlusion factor SlmA (slmA) CSB-YP385072STL
CSB-EP385072STL
CSB-BP385072STL
CSB-MP385072STL
CSB-EP385072STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L33 (rpmG) CSB-YP385073STL
CSB-EP385073STL
CSB-BP385073STL
CSB-MP385073STL
CSB-EP385073STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2-isopropylmalate synthase (leuA), partial CSB-YP385074STL
CSB-EP385074STL
CSB-BP385074STL
CSB-MP385074STL
CSB-EP385074STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP385075STL
CSB-EP385075STL
CSB-BP385075STL
CSB-MP385075STL
CSB-EP385075STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP385076STL
CSB-EP385076STL
CSB-BP385076STL
CSB-MP385076STL
CSB-EP385076STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Formate--tetrahydrofolate ligase (fhs), partial CSB-YP385077STL
CSB-EP385077STL
CSB-BP385077STL
CSB-MP385077STL
CSB-EP385077STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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