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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter baumannii N utilization substance protein B homolog (nusB) CSB-YP385397AUD
CSB-EP385397AUD
CSB-BP385397AUD
CSB-MP385397AUD
CSB-EP385397AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Imidazolonepropionase (hutI) CSB-YP385398AUD
CSB-EP385398AUD
CSB-BP385398AUD
CSB-MP385398AUD
CSB-EP385398AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Chaperone protein DnaJ (dnaJ) CSB-YP385399AUD
CSB-EP385399AUD
CSB-BP385399AUD
CSB-MP385399AUD
CSB-EP385399AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Dihydroxy-acid dehydratase (ilvD), partial CSB-YP385400AUD
CSB-EP385400AUD
CSB-BP385400AUD
CSB-MP385400AUD
CSB-EP385400AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable acetoacetate decarboxylase (adc) CSB-YP385401BPQ
CSB-EP385401BPQ
CSB-BP385401BPQ
CSB-MP385401BPQ
CSB-EP385401BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP synthase subunit beta 2 (atpD2), partial CSB-YP385402BPQ
CSB-EP385402BPQ
CSB-BP385402BPQ
CSB-MP385402BPQ
CSB-EP385402BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP385403BPQ
CSB-EP385403BPQ
CSB-BP385403BPQ
CSB-MP385403BPQ
CSB-EP385403BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Secretion apparatus protein BsaZ (bsaZ), partial CSB-YP385404BPQ1
CSB-EP385404BPQ1
CSB-BP385404BPQ1
CSB-MP385404BPQ1
CSB-EP385404BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Translocator protein BipD (bipD) CSB-YP385405BPQ
CSB-EP385405BPQ
CSB-BP385405BPQ
CSB-MP385405BPQ
CSB-EP385405BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable L-aspartate dehydrogenase (nadX) CSB-YP385406BPQ
CSB-EP385406BPQ
CSB-BP385406BPQ
CSB-MP385406BPQ
CSB-EP385406BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dictomallein (dtmL), partial CSB-YP385407BPQ
CSB-EP385407BPQ
CSB-BP385407BPQ
CSB-MP385407BPQ
CSB-EP385407BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NH (3)-dependent NAD (+) synthetase CSB-YP385408BPQ
CSB-EP385408BPQ
CSB-BP385408BPQ
CSB-MP385408BPQ
CSB-EP385408BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Indole-3-glycerol phosphate synthase (trpC) CSB-YP385409BPQ
CSB-EP385409BPQ
CSB-BP385409BPQ
CSB-MP385409BPQ
CSB-EP385409BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Kynurenine formamidase (kynB) CSB-YP385410BPQ
CSB-EP385410BPQ
CSB-BP385410BPQ
CSB-MP385410BPQ
CSB-EP385410BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S20 (rpsT) CSB-YP385411BPQ
CSB-EP385411BPQ
CSB-BP385411BPQ
CSB-MP385411BPQ
CSB-EP385411BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP385412BPQ
CSB-EP385412BPQ
CSB-BP385412BPQ
CSB-MP385412BPQ
CSB-EP385412BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA (guanine-N (1)-)-methyltransferase CSB-YP385413BPQ
CSB-EP385413BPQ
CSB-BP385413BPQ
CSB-MP385413BPQ
CSB-EP385413BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP385414BPQ
CSB-EP385414BPQ
CSB-BP385414BPQ
CSB-MP385414BPQ
CSB-EP385414BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein htpG (htpG), partial CSB-YP385415BPQ
CSB-EP385415BPQ
CSB-BP385415BPQ
CSB-MP385415BPQ
CSB-EP385415BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP385416BPQ
CSB-EP385416BPQ
CSB-BP385416BPQ
CSB-MP385416BPQ
CSB-EP385416BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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