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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei 50S ribosomal protein L16 (rplP) CSB-YP385477BPQ
CSB-EP385477BPQ
CSB-BP385477BPQ
CSB-MP385477BPQ
CSB-EP385477BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S14 (rpsN) CSB-YP385478BPQ
CSB-EP385478BPQ
CSB-BP385478BPQ
CSB-MP385478BPQ
CSB-EP385478BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L18 (rplR) CSB-YP385479BPQ
CSB-EP385479BPQ
CSB-BP385479BPQ
CSB-MP385479BPQ
CSB-EP385479BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L36 (rpmJ) CSB-YP385480BPQ
CSB-EP385480BPQ
CSB-BP385480BPQ
CSB-MP385480BPQ
CSB-EP385480BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Membrane protein insertase YidC (yidC), partial CSB-YP385481BPQ1
CSB-EP385481BPQ1
CSB-BP385481BPQ1
CSB-MP385481BPQ1
CSB-EP385481BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Proteasome subunit beta 1 (psmB1) CSB-YP385482PZV
CSB-EP385482PZV
CSB-BP385482PZV
CSB-MP385482PZV
CSB-EP385482PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Cysteine--tRNA ligase (cysS) CSB-YP385483PZV
CSB-EP385483PZV
CSB-BP385483PZV
CSB-MP385483PZV
CSB-EP385483PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Probable tRNA pseudouridine synthase B (truB) CSB-YP385484PZV
CSB-EP385484PZV
CSB-BP385484PZV
CSB-MP385484PZV
CSB-EP385484PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0215 protein Pcal_0119 (Pcal_0119) CSB-YP385485PZV
CSB-EP385485PZV
CSB-BP385485PZV
CSB-MP385485PZV
CSB-EP385485PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP385486PZV
CSB-EP385486PZV
CSB-BP385486PZV
CSB-MP385486PZV
CSB-EP385486PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Preprotein translocase subunit secG (secG), partial CSB-YP385487PZV
CSB-EP385487PZV
CSB-BP385487PZV
CSB-MP385487PZV
CSB-EP385487PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Homoserine kinase (thrB) CSB-YP385488PZV
CSB-EP385488PZV
CSB-BP385488PZV
CSB-MP385488PZV
CSB-EP385488PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Histidine--tRNA ligase (hisS) CSB-YP385489PZV
CSB-EP385489PZV
CSB-BP385489PZV
CSB-MP385489PZV
CSB-EP385489PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0201 protein Pcal_0593 (Pcal_0593) CSB-YP385490PZV
CSB-EP385490PZV
CSB-BP385490PZV
CSB-MP385490PZV
CSB-EP385490PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S5P (rps5p) CSB-YP385491PZV
CSB-EP385491PZV
CSB-BP385491PZV
CSB-MP385491PZV
CSB-EP385491PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S6e (rps6e) CSB-YP385492PZV
CSB-EP385492PZV
CSB-BP385492PZV
CSB-MP385492PZV
CSB-EP385492PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 3-dehydroquinate synthase (aroB) CSB-YP385493PZV
CSB-EP385493PZV
CSB-BP385493PZV
CSB-MP385493PZV
CSB-EP385493PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S13P (rps13p) CSB-YP385494PZV
CSB-EP385494PZV
CSB-BP385494PZV
CSB-MP385494PZV
CSB-EP385494PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Probable DNA primase small subunit (priA) CSB-YP385495PZV
CSB-EP385495PZV
CSB-BP385495PZV
CSB-MP385495PZV
CSB-EP385495PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0173 metal-dependent hydrolase Pcal_1074 (Pcal_1074) CSB-YP385496PZV
CSB-EP385496PZV
CSB-BP385496PZV
CSB-MP385496PZV
CSB-EP385496PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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