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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Scheffersomyces stipitis Cytochrome oxidase assembly protein 3, mitochondrial (COA3), partial CSB-YP385317SXM1
CSB-EP385317SXM1
CSB-BP385317SXM1
CSB-MP385317SXM1
CSB-EP385317SXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis GDP-mannose transporter (VRG4), partial CSB-YP385318SXM
CSB-EP385318SXM
CSB-BP385318SXM
CSB-MP385318SXM
CSB-EP385318SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ATPase GET3 (GET3) CSB-YP385319SXM
CSB-EP385319SXM
CSB-BP385319SXM
CSB-MP385319SXM
CSB-EP385319SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Autophagy-related protein 17 (ATG17) CSB-YP385320SXM
CSB-EP385320SXM
CSB-BP385320SXM
CSB-MP385320SXM
CSB-EP385320SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Autophagy-related protein 3 (ATG3) CSB-YP385321SXM
CSB-EP385321SXM
CSB-BP385321SXM
CSB-MP385321SXM
CSB-EP385321SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Ribosome biogenesis protein ERB1 (ERB1), partial CSB-YP385322SXM
CSB-EP385322SXM
CSB-BP385322SXM
CSB-MP385322SXM
CSB-EP385322SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4) CSB-YP385323SXM
CSB-EP385323SXM
CSB-BP385323SXM
CSB-MP385323SXM
CSB-EP385323SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Signal sequence processing protein (SEC11), partial CSB-YP385324SXM
CSB-EP385324SXM
CSB-BP385324SXM
CSB-MP385324SXM
CSB-EP385324SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis 3-ketoacyl-CoA reductase (PICST_79198), partial CSB-YP385325SXM1
CSB-EP385325SXM1
CSB-BP385325SXM1
CSB-MP385325SXM1
CSB-EP385325SXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mitochondrial inner membrane protease ATP23 (ATP23) CSB-YP385326SXM
CSB-EP385326SXM
CSB-BP385326SXM
CSB-MP385326SXM
CSB-EP385326SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Lysophospholipase NTE1 (NTE1), partial CSB-YP385327SXM
CSB-EP385327SXM
CSB-BP385327SXM
CSB-MP385327SXM
CSB-EP385327SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ADP-ribose 1'-phosphate phosphatase (POA1) CSB-YP385328SXM
CSB-EP385328SXM
CSB-BP385328SXM
CSB-MP385328SXM
CSB-EP385328SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Autophagy-related protein 9 (ATG9), partial CSB-YP385329SXM
CSB-EP385329SXM
CSB-BP385329SXM
CSB-MP385329SXM
CSB-EP385329SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Histone H2A.1 (HTA2) CSB-YP385330SXM
CSB-EP385330SXM
CSB-BP385330SXM
CSB-MP385330SXM
CSB-EP385330SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mediator of RNA polymerase II transcription subunit 5 (NUT1), partial CSB-YP385331SXM
CSB-EP385331SXM
CSB-BP385331SXM
CSB-MP385331SXM
CSB-EP385331SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Riboflavin kinase (FMN1) CSB-YP385332SXM
CSB-EP385332SXM
CSB-BP385332SXM
CSB-MP385332SXM
CSB-EP385332SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Ribosome biogenesis protein ALB1 (ALB1) CSB-YP385333SXM
CSB-EP385333SXM
CSB-BP385333SXM
CSB-MP385333SXM
CSB-EP385333SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii DNA replication and repair protein recF (recF) CSB-YP385334AUD
CSB-EP385334AUD
CSB-BP385334AUD
CSB-MP385334AUD
CSB-EP385334AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii D-amino acid dehydrogenase small subunit (dadA) CSB-YP385335AUD
CSB-EP385335AUD
CSB-BP385335AUD
CSB-MP385335AUD
CSB-EP385335AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii ATP synthase gamma chain (atpG) CSB-YP385336AUD
CSB-EP385336AUD
CSB-BP385336AUD
CSB-MP385336AUD
CSB-EP385336AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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