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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP385517AUF
CSB-EP385517AUF
CSB-BP385517AUF
CSB-MP385517AUF
CSB-EP385517AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP385518AUF
CSB-EP385518AUF
CSB-BP385518AUF
CSB-MP385518AUF
CSB-EP385518AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b D-alanine--D-alanine ligase (ddl) CSB-YP385519AUF
CSB-EP385519AUF
CSB-BP385519AUF
CSB-MP385519AUF
CSB-EP385519AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b GTPase obg (obg) CSB-YP385520AUF
CSB-EP385520AUF
CSB-BP385520AUF
CSB-MP385520AUF
CSB-EP385520AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Recombination protein RecR (recR) CSB-YP385521AUF
CSB-EP385521AUF
CSB-BP385521AUF
CSB-MP385521AUF
CSB-EP385521AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Dihydroxy-acid dehydratase (ilvD), partial CSB-YP385522AUF
CSB-EP385522AUF
CSB-BP385522AUF
CSB-MP385522AUF
CSB-EP385522AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribonuclease HII (rnhB) CSB-YP385523AUF
CSB-EP385523AUF
CSB-BP385523AUF
CSB-MP385523AUF
CSB-EP385523AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP385524AUF
CSB-EP385524AUF
CSB-BP385524AUF
CSB-MP385524AUF
CSB-EP385524AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP385525AUF1
CSB-EP385525AUF1
CSB-BP385525AUF1
CSB-MP385525AUF1
CSB-EP385525AUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Electron transport complex protein RnfD (rnfD), partial CSB-YP385526AUF
CSB-EP385526AUF
CSB-BP385526AUF
CSB-MP385526AUF
CSB-EP385526AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b N utilization substance protein B homolog (nusB) CSB-YP385527AUF
CSB-EP385527AUF
CSB-BP385527AUF
CSB-MP385527AUF
CSB-EP385527AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Protein translocase subunit SecA (secA), partial CSB-YP385528AUF
CSB-EP385528AUF
CSB-BP385528AUF
CSB-MP385528AUF
CSB-EP385528AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP385529AUF
CSB-EP385529AUF
CSB-BP385529AUF
CSB-MP385529AUF
CSB-EP385529AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Na (+)/H (+) antiporter nhaB, partial CSB-YP385530AUF
CSB-EP385530AUF
CSB-BP385530AUF
CSB-MP385530AUF
CSB-EP385530AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP385531AUF
CSB-EP385531AUF
CSB-BP385531AUF
CSB-MP385531AUF
CSB-EP385531AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Methionine--tRNA ligase (metG), partial CSB-YP385532AUF
CSB-EP385532AUF
CSB-BP385532AUF
CSB-MP385532AUF
CSB-EP385532AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Autonomous glycyl radical cofactor (grcA) CSB-YP385533AUF
CSB-EP385533AUF
CSB-BP385533AUF
CSB-MP385533AUF
CSB-EP385533AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0756 membrane protein APL_0366 (APL_0366), partial CSB-YP385534AUF1
CSB-EP385534AUF1
CSB-BP385534AUF1
CSB-MP385534AUF1
CSB-EP385534AUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP385535AUF
CSB-EP385535AUF
CSB-BP385535AUF
CSB-MP385535AUF
CSB-EP385535AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Uracil phosphoribosyltransferase (upp) CSB-YP385536AUF
CSB-EP385536AUF
CSB-BP385536AUF
CSB-MP385536AUF
CSB-EP385536AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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