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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica UPF0042 nucleotide-binding protein Sbal_3671 (Sbal_3671) CSB-YP386338STL
CSB-EP386338STL
CSB-BP386338STL
CSB-MP386338STL
CSB-EP386338STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP386339STL
CSB-EP386339STL
CSB-BP386339STL
CSB-MP386339STL
CSB-EP386339STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP386340STL
CSB-EP386340STL
CSB-BP386340STL
CSB-MP386340STL
CSB-EP386340STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA gyrase inhibitor YacG (yacG) CSB-YP386341STL
CSB-EP386341STL
CSB-BP386341STL
CSB-MP386341STL
CSB-EP386341STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0061 protein Sbal_4063 (Sbal_4063) CSB-YP386342STL
CSB-EP386342STL
CSB-BP386342STL
CSB-MP386342STL
CSB-EP386342STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Shikimate kinase (aroK) CSB-YP386343STL
CSB-EP386343STL
CSB-BP386343STL
CSB-MP386343STL
CSB-EP386343STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP386344STL
CSB-EP386344STL
CSB-BP386344STL
CSB-MP386344STL
CSB-EP386344STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L17 (rplQ) CSB-YP386345STL
CSB-EP386345STL
CSB-BP386345STL
CSB-MP386345STL
CSB-EP386345STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L36 (rpmJ) CSB-YP386346STL
CSB-EP386346STL
CSB-BP386346STL
CSB-MP386346STL
CSB-EP386346STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S5 (rpsE) CSB-YP386347STL
CSB-EP386347STL
CSB-BP386347STL
CSB-MP386347STL
CSB-EP386347STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S14 (rpsN) CSB-YP386348STL
CSB-EP386348STL
CSB-BP386348STL
CSB-MP386348STL
CSB-EP386348STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L5 (rplE) CSB-YP386349STL
CSB-EP386349STL
CSB-BP386349STL
CSB-MP386349STL
CSB-EP386349STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L29 (rpmC) CSB-YP386350STL
CSB-EP386350STL
CSB-BP386350STL
CSB-MP386350STL
CSB-EP386350STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA 2-selenouridine synthase (selU) CSB-YP386351STL
CSB-EP386351STL
CSB-BP386351STL
CSB-MP386351STL
CSB-EP386351STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein-export protein secB (secB) CSB-YP386352STL
CSB-EP386352STL
CSB-BP386352STL
CSB-MP386352STL
CSB-EP386352STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase gamma chain (atpG) CSB-YP386353STL
CSB-EP386353STL
CSB-BP386353STL
CSB-MP386353STL
CSB-EP386353STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP386354STL
CSB-EP386354STL
CSB-BP386354STL
CSB-MP386354STL
CSB-EP386354STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Membrane protein insertase YidC (yidC), partial CSB-YP386355STL1
CSB-EP386355STL1
CSB-BP386355STL1
CSB-MP386355STL1
CSB-EP386355STL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Elongation factor Tu 2 (tuf2) CSB-YP386356STL
CSB-EP386356STL
CSB-BP386356STL
CSB-MP386356STL
CSB-EP386356STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Hydroxylamine reductase (hcp), partial CSB-YP386357DUT
CSB-EP386357DUT
CSB-BP386357DUT
CSB-MP386357DUT
CSB-EP386357DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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