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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methylibium petroleiphilum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP385917MNX
CSB-EP385917MNX
CSB-BP385917MNX
CSB-MP385917MNX
CSB-EP385917MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L21 (rplU) CSB-YP385918MNX
CSB-EP385918MNX
CSB-BP385918MNX
CSB-MP385918MNX
CSB-EP385918MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP385919MNX
CSB-EP385919MNX
CSB-BP385919MNX
CSB-MP385919MNX
CSB-EP385919MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L32 (rpmF) CSB-YP385920MNX
CSB-EP385920MNX
CSB-BP385920MNX
CSB-MP385920MNX
CSB-EP385920MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Urease accessory protein UreF (ureF) CSB-YP385921MNX
CSB-EP385921MNX
CSB-BP385921MNX
CSB-MP385921MNX
CSB-EP385921MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Urease subunit beta (ureB) CSB-YP385922MNX
CSB-EP385922MNX
CSB-BP385922MNX
CSB-MP385922MNX
CSB-EP385922MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Histidinol-phosphate aminotransferase (hisC) CSB-YP385923MNX
CSB-EP385923MNX
CSB-BP385923MNX
CSB-MP385923MNX
CSB-EP385923MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP385924MNX
CSB-EP385924MNX
CSB-BP385924MNX
CSB-MP385924MNX
CSB-EP385924MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP385925MNX
CSB-EP385925MNX
CSB-BP385925MNX
CSB-MP385925MNX
CSB-EP385925MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S16 (rpsP) CSB-YP385926MNX
CSB-EP385926MNX
CSB-BP385926MNX
CSB-MP385926MNX
CSB-EP385926MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribosome maturation factor RimM (rimM) CSB-YP385927MNX
CSB-EP385927MNX
CSB-BP385927MNX
CSB-MP385927MNX
CSB-EP385927MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Outer-membrane lipoprotein carrier protein (lolA) CSB-YP385928MNX
CSB-EP385928MNX
CSB-BP385928MNX
CSB-MP385928MNX
CSB-EP385928MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 3-methyl-2-oxobutanoate hydroxymethyltransferase 2 (panB2) CSB-YP385929MNX
CSB-EP385929MNX
CSB-BP385929MNX
CSB-MP385929MNX
CSB-EP385929MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 5'-nucleotidase surE (surE) CSB-YP385930MNX
CSB-EP385930MNX
CSB-BP385930MNX
CSB-MP385930MNX
CSB-EP385930MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Phosphoglucosamine mutase (glmM) CSB-YP385931MNX
CSB-EP385931MNX
CSB-BP385931MNX
CSB-MP385931MNX
CSB-EP385931MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP385932MNX
CSB-EP385932MNX
CSB-BP385932MNX
CSB-MP385932MNX
CSB-EP385932MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP385933MNX
CSB-EP385933MNX
CSB-BP385933MNX
CSB-MP385933MNX
CSB-EP385933MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP385934MNX
CSB-EP385934MNX
CSB-BP385934MNX
CSB-MP385934MNX
CSB-EP385934MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum UPF0502 protein Mpe_A1449 (Mpe_A1449) CSB-YP385935MNX
CSB-EP385935MNX
CSB-BP385935MNX
CSB-MP385935MNX
CSB-EP385935MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Fructose-1,6-bisphosphatase class 1 1 (fbp1) CSB-YP385936MNX
CSB-EP385936MNX
CSB-BP385936MNX
CSB-MP385936MNX
CSB-EP385936MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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