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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter baumannii Phosphomethylpyrimidine synthase (thiC), partial CSB-YP386518AUD
CSB-EP386518AUD
CSB-BP386518AUD
CSB-MP386518AUD
CSB-EP386518AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L11 (rplK) CSB-YP386519AUD
CSB-EP386519AUD
CSB-BP386519AUD
CSB-MP386519AUD
CSB-EP386519AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L10 (rplJ) CSB-YP386520AUD
CSB-EP386520AUD
CSB-BP386520AUD
CSB-MP386520AUD
CSB-EP386520AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosome maturation factor RimP (rimP) CSB-YP386521AUD
CSB-EP386521AUD
CSB-BP386521AUD
CSB-MP386521AUD
CSB-EP386521AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Protein CrcB homolog (crcB), partial CSB-YP386522AUD1
CSB-EP386522AUD1
CSB-BP386522AUD1
CSB-MP386522AUD1
CSB-EP386522AUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP386523AUD
CSB-EP386523AUD
CSB-BP386523AUD
CSB-MP386523AUD
CSB-EP386523AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Translation initiation factor IF-1 (infA) CSB-YP386524AUD
CSB-EP386524AUD
CSB-BP386524AUD
CSB-MP386524AUD
CSB-EP386524AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP386525AUD1
CSB-EP386525AUD1
CSB-BP386525AUD1
CSB-MP386525AUD1
CSB-EP386525AUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Homoserine O-acetyltransferase (metX) CSB-YP386526AUD
CSB-EP386526AUD
CSB-BP386526AUD
CSB-MP386526AUD
CSB-EP386526AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Gamma-glutamyl phosphate reductase (proA) CSB-YP386527AUD
CSB-EP386527AUD
CSB-BP386527AUD
CSB-MP386527AUD
CSB-EP386527AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP386528AUD
CSB-EP386528AUD
CSB-BP386528AUD
CSB-MP386528AUD
CSB-EP386528AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Protein-export protein secB (secB) CSB-YP386529AUD
CSB-EP386529AUD
CSB-BP386529AUD
CSB-MP386529AUD
CSB-EP386529AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Pantothenate synthetase (panC) CSB-YP386530AUD
CSB-EP386530AUD
CSB-BP386530AUD
CSB-MP386530AUD
CSB-EP386530AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L20 (rplT) CSB-YP386531AUD
CSB-EP386531AUD
CSB-BP386531AUD
CSB-MP386531AUD
CSB-EP386531AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Histidinol-phosphate aminotransferase (hisC) CSB-YP386532AUD
CSB-EP386532AUD
CSB-BP386532AUD
CSB-MP386532AUD
CSB-EP386532AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Darcynin 2 (A1S_0743) CSB-YP386533AUD
CSB-EP386533AUD
CSB-BP386533AUD
CSB-MP386533AUD
CSB-EP386533AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP386534AUD
CSB-EP386534AUD
CSB-BP386534AUD
CSB-MP386534AUD
CSB-EP386534AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Deoxycytidine triphosphate deaminase (dcd) CSB-YP386535AUD
CSB-EP386535AUD
CSB-BP386535AUD
CSB-MP386535AUD
CSB-EP386535AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii DNA ligase (ligA), partial CSB-YP386536AUD
CSB-EP386536AUD
CSB-BP386536AUD
CSB-MP386536AUD
CSB-EP386536AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L25 (rplY) CSB-YP386537AUD
CSB-EP386537AUD
CSB-BP386537AUD
CSB-MP386537AUD
CSB-EP386537AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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