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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium thermocellum Probable septum site-determining protein MinC (minC) CSB-YP387518DUT
CSB-EP387518DUT
CSB-BP387518DUT
CSB-MP387518DUT
CSB-EP387518DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA (guanine-N (7)-)-methyltransferase CSB-YP387519DUT
CSB-EP387519DUT
CSB-BP387519DUT
CSB-MP387519DUT
CSB-EP387519DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Putative sporulation transcription regulator WhiA (whiA) CSB-YP387520DUT
CSB-EP387520DUT
CSB-BP387520DUT
CSB-MP387520DUT
CSB-EP387520DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum GTPase obg (obg) CSB-YP387521DUT
CSB-EP387521DUT
CSB-BP387521DUT
CSB-MP387521DUT
CSB-EP387521DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP387522DUT
CSB-EP387522DUT
CSB-BP387522DUT
CSB-MP387522DUT
CSB-EP387522DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S15 (rpsO) CSB-YP387523DUT
CSB-EP387523DUT
CSB-BP387523DUT
CSB-MP387523DUT
CSB-EP387523DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Phosphopentomutase (deoB) CSB-YP387524DUT
CSB-EP387524DUT
CSB-BP387524DUT
CSB-MP387524DUT
CSB-EP387524DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Tyrosine--tRNA ligase (tyrS) CSB-YP387525DUT
CSB-EP387525DUT
CSB-BP387525DUT
CSB-MP387525DUT
CSB-EP387525DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribonuclease HII (rnhB) CSB-YP387526DUT
CSB-EP387526DUT
CSB-BP387526DUT
CSB-MP387526DUT
CSB-EP387526DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum LexA repressor (lexA) CSB-YP387527DUT
CSB-EP387527DUT
CSB-BP387527DUT
CSB-MP387527DUT
CSB-EP387527DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP387528DUT
CSB-EP387528DUT
CSB-BP387528DUT
CSB-MP387528DUT
CSB-EP387528DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum N utilization substance protein B homolog (nusB) CSB-YP387529DUT
CSB-EP387529DUT
CSB-BP387529DUT
CSB-MP387529DUT
CSB-EP387529DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0365 protein Cthe_0858 (Cthe_0858), partial CSB-YP387530DUT1
CSB-EP387530DUT1
CSB-BP387530DUT1
CSB-MP387530DUT1
CSB-EP387530DUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Anthranilate phosphoribosyltransferase (trpD) CSB-YP387531DUT
CSB-EP387531DUT
CSB-BP387531DUT
CSB-MP387531DUT
CSB-EP387531DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP387532DUT
CSB-EP387532DUT
CSB-BP387532DUT
CSB-MP387532DUT
CSB-EP387532DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Dihydroorotase (pyrC) CSB-YP387533DUT
CSB-EP387533DUT
CSB-BP387533DUT
CSB-MP387533DUT
CSB-EP387533DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP387534DUT
CSB-EP387534DUT
CSB-BP387534DUT
CSB-MP387534DUT
CSB-EP387534DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP387535DUT
CSB-EP387535DUT
CSB-BP387535DUT
CSB-MP387535DUT
CSB-EP387535DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP387536DUT
CSB-EP387536DUT
CSB-BP387536DUT
CSB-MP387536DUT
CSB-EP387536DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S2 (rpsB) CSB-YP387537DUT
CSB-EP387537DUT
CSB-BP387537DUT
CSB-MP387537DUT
CSB-EP387537DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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