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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methylibium petroleiphilum 30S ribosomal protein S20 (rpsT) CSB-YP385957MNX
CSB-EP385957MNX
CSB-BP385957MNX
CSB-MP385957MNX
CSB-EP385957MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum UPF0234 protein Mpe_A3039 (Mpe_A3039) CSB-YP385958MNX
CSB-EP385958MNX
CSB-BP385958MNX
CSB-MP385958MNX
CSB-EP385958MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S9 (rpsI) CSB-YP385959MNX
CSB-EP385959MNX
CSB-BP385959MNX
CSB-MP385959MNX
CSB-EP385959MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP385960MNX
CSB-EP385960MNX
CSB-BP385960MNX
CSB-MP385960MNX
CSB-EP385960MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP385961MNX
CSB-EP385961MNX
CSB-BP385961MNX
CSB-MP385961MNX
CSB-EP385961MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum S-adenosylmethionine synthase (metK) CSB-YP385962MNX
CSB-EP385962MNX
CSB-BP385962MNX
CSB-MP385962MNX
CSB-EP385962MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Acetaldehyde dehydrogenase 2 (Mpe_A3322) CSB-YP385963MNX
CSB-EP385963MNX
CSB-BP385963MNX
CSB-MP385963MNX
CSB-EP385963MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP385964MNX
CSB-EP385964MNX
CSB-BP385964MNX
CSB-MP385964MNX
CSB-EP385964MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Type III pantothenate kinase (coaX) CSB-YP385965MNX
CSB-EP385965MNX
CSB-BP385965MNX
CSB-MP385965MNX
CSB-EP385965MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S4 (rpsD) CSB-YP385966MNX
CSB-EP385966MNX
CSB-BP385966MNX
CSB-MP385966MNX
CSB-EP385966MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L30 (rpmD) CSB-YP385967MNX
CSB-EP385967MNX
CSB-BP385967MNX
CSB-MP385967MNX
CSB-EP385967MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L18 (rplR) CSB-YP385968MNX
CSB-EP385968MNX
CSB-BP385968MNX
CSB-MP385968MNX
CSB-EP385968MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L14 (rplN) CSB-YP385969MNX
CSB-EP385969MNX
CSB-BP385969MNX
CSB-MP385969MNX
CSB-EP385969MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L29 (rpmC) CSB-YP385970MNX
CSB-EP385970MNX
CSB-BP385970MNX
CSB-MP385970MNX
CSB-EP385970MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L2 (rplB) CSB-YP385971MNX
CSB-EP385971MNX
CSB-BP385971MNX
CSB-MP385971MNX
CSB-EP385971MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S12 (rpsL) CSB-YP385972MNX
CSB-EP385972MNX
CSB-BP385972MNX
CSB-MP385972MNX
CSB-EP385972MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L10 (rplJ) CSB-YP385973MNX
CSB-EP385973MNX
CSB-BP385973MNX
CSB-MP385973MNX
CSB-EP385973MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L1 (rplA) CSB-YP385974MNX
CSB-EP385974MNX
CSB-BP385974MNX
CSB-MP385974MNX
CSB-EP385974MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Aspartate 1-decarboxylase (panD) CSB-YP385975MNX
CSB-EP385975MNX
CSB-BP385975MNX
CSB-MP385975MNX
CSB-EP385975MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum PKHD-type hydroxylase Mpe_A3725 (Mpe_A3725) CSB-YP385976MNX
CSB-EP385976MNX
CSB-BP385976MNX
CSB-MP385976MNX
CSB-EP385976MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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