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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica Acetate kinase (ackA) CSB-YP386318STL
CSB-EP386318STL
CSB-BP386318STL
CSB-MP386318STL
CSB-EP386318STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Tryptophan synthase alpha chain (trpA) CSB-YP386319STL
CSB-EP386319STL
CSB-BP386319STL
CSB-MP386319STL
CSB-EP386319STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0115 protein Sbal_2754 (Sbal_2754) CSB-YP386320STL
CSB-EP386320STL
CSB-BP386320STL
CSB-MP386320STL
CSB-EP386320STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP386321STL
CSB-EP386321STL
CSB-BP386321STL
CSB-MP386321STL
CSB-EP386321STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP386322STL
CSB-EP386322STL
CSB-BP386322STL
CSB-MP386322STL
CSB-EP386322STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable Fe (2+)-trafficking protein CSB-YP386323STL
CSB-EP386323STL
CSB-BP386323STL
CSB-MP386323STL
CSB-EP386323STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Carbon storage regulator homolog (csrA) CSB-YP386324STL
CSB-EP386324STL
CSB-BP386324STL
CSB-MP386324STL
CSB-EP386324STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP386325STL
CSB-EP386325STL
CSB-BP386325STL
CSB-MP386325STL
CSB-EP386325STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP386326STL
CSB-EP386326STL
CSB-BP386326STL
CSB-MP386326STL
CSB-EP386326STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Transcriptional repressor NrdR (nrdR) CSB-YP386327STL
CSB-EP386327STL
CSB-BP386327STL
CSB-MP386327STL
CSB-EP386327STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphopentomutase (deoB) CSB-YP386328STL
CSB-EP386328STL
CSB-BP386328STL
CSB-MP386328STL
CSB-EP386328STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Peptide chain release factor 3 (prfC), partial CSB-YP386329STL
CSB-EP386329STL
CSB-BP386329STL
CSB-MP386329STL
CSB-EP386329STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosome-binding factor A (rbfA) CSB-YP386330STL
CSB-EP386330STL
CSB-BP386330STL
CSB-MP386330STL
CSB-EP386330STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP386331STL
CSB-EP386331STL
CSB-BP386331STL
CSB-MP386331STL
CSB-EP386331STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP386332STL
CSB-EP386332STL
CSB-BP386332STL
CSB-MP386332STL
CSB-EP386332STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Leucine--tRNA ligase (leuS), partial CSB-YP386333STL
CSB-EP386333STL
CSB-BP386333STL
CSB-MP386333STL
CSB-EP386333STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chaperone protein DnaJ (dnaJ) CSB-YP386334STL
CSB-EP386334STL
CSB-BP386334STL
CSB-MP386334STL
CSB-EP386334STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP386335STL
CSB-EP386335STL
CSB-BP386335STL
CSB-MP386335STL
CSB-EP386335STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica PKHD-type hydroxylase Sbal_3634 (Sbal_3634) CSB-YP386336STL
CSB-EP386336STL
CSB-BP386336STL
CSB-MP386336STL
CSB-EP386336STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L13 (rplM) CSB-YP386337STL
CSB-EP386337STL
CSB-BP386337STL
CSB-MP386337STL
CSB-EP386337STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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