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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum Formate--tetrahydrofolate ligase (fhs), partial CSB-YP387558DUT
CSB-EP387558DUT
CSB-BP387558DUT
CSB-MP387558DUT
CSB-EP387558DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ketol-acid reductoisomerase (ilvC) CSB-YP387559DUT
CSB-EP387559DUT
CSB-BP387559DUT
CSB-MP387559DUT
CSB-EP387559DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Type III pantothenate kinase (coaX) CSB-YP387560DUT
CSB-EP387560DUT
CSB-BP387560DUT
CSB-MP387560DUT
CSB-EP387560DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP synthase epsilon chain (atpC) CSB-YP387561DUT
CSB-EP387561DUT
CSB-BP387561DUT
CSB-MP387561DUT
CSB-EP387561DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L33 (rpmG) CSB-YP387562DUT
CSB-EP387562DUT
CSB-BP387562DUT
CSB-MP387562DUT
CSB-EP387562DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L1 (rplA) CSB-YP387563DUT
CSB-EP387563DUT
CSB-BP387563DUT
CSB-MP387563DUT
CSB-EP387563DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP387564DUT
CSB-EP387564DUT
CSB-BP387564DUT
CSB-MP387564DUT
CSB-EP387564DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP387565DUT
CSB-EP387565DUT
CSB-BP387565DUT
CSB-MP387565DUT
CSB-EP387565DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP387566DUT
CSB-EP387566DUT
CSB-BP387566DUT
CSB-MP387566DUT
CSB-EP387566DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S10 (rpsJ) CSB-YP387567DUT
CSB-EP387567DUT
CSB-BP387567DUT
CSB-MP387567DUT
CSB-EP387567DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L2 (rplB) CSB-YP387568DUT
CSB-EP387568DUT
CSB-BP387568DUT
CSB-MP387568DUT
CSB-EP387568DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L29 (rpmC) CSB-YP387569DUT
CSB-EP387569DUT
CSB-BP387569DUT
CSB-MP387569DUT
CSB-EP387569DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S14 type Z (rpsZ) CSB-YP387570DUT
CSB-EP387570DUT
CSB-BP387570DUT
CSB-MP387570DUT
CSB-EP387570DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S5 (rpsE) CSB-YP387571DUT
CSB-EP387571DUT
CSB-BP387571DUT
CSB-MP387571DUT
CSB-EP387571DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L36 (rpmJ) CSB-YP387572DUT
CSB-EP387572DUT
CSB-BP387572DUT
CSB-MP387572DUT
CSB-EP387572DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S11 (rpsK) CSB-YP387573DUT
CSB-EP387573DUT
CSB-BP387573DUT
CSB-MP387573DUT
CSB-EP387573DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 7-cyano-7-deazaguanine synthase (queC) CSB-YP387574DUT
CSB-EP387574DUT
CSB-BP387574DUT
CSB-MP387574DUT
CSB-EP387574DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Cobalamin synthase (cobS), partial CSB-YP387575DUT1
CSB-EP387575DUT1
CSB-BP387575DUT1
CSB-MP387575DUT1
CSB-EP387575DUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Probable peroxiredoxin (Smar_0058) CSB-YP387576SUP
CSB-EP387576SUP
CSB-BP387576SUP
CSB-MP387576SUP
CSB-EP387576SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Uncharacterized solute-binding protein Smar_0280 (Smar_0280) CSB-YP387577SUP
CSB-EP387577SUP
CSB-BP387577SUP
CSB-MP387577SUP
CSB-EP387577SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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