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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP386358DUT
CSB-EP386358DUT
CSB-BP386358DUT
CSB-MP386358DUT
CSB-EP386358DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP386359DUT
CSB-EP386359DUT
CSB-BP386359DUT
CSB-MP386359DUT
CSB-EP386359DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L21 (rplU) CSB-YP386360DUT
CSB-EP386360DUT
CSB-BP386360DUT
CSB-MP386360DUT
CSB-EP386360DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP386361DUT
CSB-EP386361DUT
CSB-BP386361DUT
CSB-MP386361DUT
CSB-EP386361DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP386362DUT
CSB-EP386362DUT
CSB-BP386362DUT
CSB-MP386362DUT
CSB-EP386362DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0272 protein Cthe_0369 (Cthe_0369) CSB-YP386363DUT
CSB-EP386363DUT
CSB-BP386363DUT
CSB-MP386363DUT
CSB-EP386363DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0210 protein Cthe_0410 (Cthe_0410) CSB-YP386364DUT
CSB-EP386364DUT
CSB-BP386364DUT
CSB-MP386364DUT
CSB-EP386364DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP386365DUT
CSB-EP386365DUT
CSB-BP386365DUT
CSB-MP386365DUT
CSB-EP386365DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Thiazole synthase (thiG) CSB-YP386366DUT
CSB-EP386366DUT
CSB-BP386366DUT
CSB-MP386366DUT
CSB-EP386366DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glutamate--tRNA ligase (gltX), partial CSB-YP386367DUT
CSB-EP386367DUT
CSB-BP386367DUT
CSB-MP386367DUT
CSB-EP386367DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Chorismate synthase (aroC) CSB-YP386368DUT
CSB-EP386368DUT
CSB-BP386368DUT
CSB-MP386368DUT
CSB-EP386368DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0102 protein Cthe_0758 (Cthe_0758) CSB-YP386369DUT
CSB-EP386369DUT
CSB-BP386369DUT
CSB-MP386369DUT
CSB-EP386369DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosome maturation factor RimM (rimM) CSB-YP386370DUT
CSB-EP386370DUT
CSB-BP386370DUT
CSB-MP386370DUT
CSB-EP386370DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP386371DUT
CSB-EP386371DUT
CSB-BP386371DUT
CSB-MP386371DUT
CSB-EP386371DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Arginine repressor (argR) CSB-YP386372DUT
CSB-EP386372DUT
CSB-BP386372DUT
CSB-MP386372DUT
CSB-EP386372DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Indole-3-glycerol phosphate synthase (trpC) CSB-YP386373DUT
CSB-EP386373DUT
CSB-BP386373DUT
CSB-MP386373DUT
CSB-EP386373DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Lipoprotein signal peptidase (lspA), partial CSB-YP386374DUT1
CSB-EP386374DUT1
CSB-BP386374DUT1
CSB-MP386374DUT1
CSB-EP386374DUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP386375DUT
CSB-EP386375DUT
CSB-BP386375DUT
CSB-MP386375DUT
CSB-EP386375DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Bifunctional protein pyrR (pyrR) CSB-YP386376DUT
CSB-EP386376DUT
CSB-BP386376DUT
CSB-MP386376DUT
CSB-EP386376DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Dihydrodipicolinate synthase (dapA) CSB-YP386377DUT
CSB-EP386377DUT
CSB-BP386377DUT
CSB-MP386377DUT
CSB-EP386377DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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