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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. indica GTP-binding nuclear protein Ran-1 (RAN1) CSB-YP386057OFF
CSB-EP386057OFF
CSB-BP386057OFF
CSB-MP386057OFF
CSB-EP386057OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Auxin-responsive protein IAA5 (IAA5) CSB-YP386058OFF
CSB-EP386058OFF
CSB-BP386058OFF
CSB-MP386058OFF
CSB-EP386058OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Transcription factor GAMYB (GAM1), partial CSB-YP386059OFF
CSB-EP386059OFF
CSB-BP386059OFF
CSB-MP386059OFF
CSB-EP386059OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Fructose-1,6-bisphosphatase, cytosolic (OsI_04558) CSB-YP386060OFF
CSB-EP386060OFF
CSB-BP386060OFF
CSB-MP386060OFF
CSB-EP386060OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein terminal ear1 homolog (PLA2), partial CSB-YP386061OFF
CSB-EP386061OFF
CSB-BP386061OFF
CSB-MP386061OFF
CSB-EP386061OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable sucrose-phosphate synthase 1 (SPS1), partial CSB-YP386062OFF
CSB-EP386062OFF
CSB-BP386062OFF
CSB-MP386062OFF
CSB-EP386062OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Squamosa promoter-binding-like protein 3 (SPL3) CSB-YP386063OFF
CSB-EP386063OFF
CSB-BP386063OFF
CSB-MP386063OFF
CSB-EP386063OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Beta-galactosidase 4 (OsI_006270), partial CSB-YP386064OFF
CSB-EP386064OFF
CSB-BP386064OFF
CSB-MP386064OFF
CSB-EP386064OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica CASP-like protein OsI_06397 (OsI_06397), partial CSB-YP386065OFF1
CSB-EP386065OFF1
CSB-BP386065OFF1
CSB-MP386065OFF1
CSB-EP386065OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Bidirectional sugar transporter SWEET15 (SWEET15), partial CSB-YP386066OFF1
CSB-EP386066OFF1
CSB-BP386066OFF1
CSB-MP386066OFF1
CSB-EP386066OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cyclin-dependent kinase G-1 (CDKG-1), partial CSB-YP386067OFF
CSB-EP386067OFF
CSB-BP386067OFF
CSB-MP386067OFF
CSB-EP386067OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica SPX domain-containing membrane protein OsI_08463 (OsI_08463), partial CSB-YP386068OFF
CSB-EP386068OFF
CSB-BP386068OFF
CSB-MP386068OFF
CSB-EP386068OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX16 (HOX16) CSB-YP386069OFF
CSB-EP386069OFF
CSB-BP386069OFF
CSB-MP386069OFF
CSB-EP386069OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX10 (HOX10), partial CSB-YP386070OFF
CSB-EP386070OFF
CSB-BP386070OFF
CSB-MP386070OFF
CSB-EP386070OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Putative laccase-18 (LAC18), partial CSB-YP386071OFF
CSB-EP386071OFF
CSB-BP386071OFF
CSB-MP386071OFF
CSB-EP386071OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX21 (HOX21) CSB-YP386072OFF
CSB-EP386072OFF
CSB-BP386072OFF
CSB-MP386072OFF
CSB-EP386072OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX13 (HOX13) CSB-YP386073OFF
CSB-EP386073OFF
CSB-BP386073OFF
CSB-MP386073OFF
CSB-EP386073OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX12 (HOX12) CSB-YP386074OFF
CSB-EP386074OFF
CSB-BP386074OFF
CSB-MP386074OFF
CSB-EP386074OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Two-component response regulator-like PRR73 (PRR73), partial CSB-YP386075OFF
CSB-EP386075OFF
CSB-BP386075OFF
CSB-MP386075OFF
CSB-EP386075OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Peroxisomal membrane protein 11-3 (PEX11-3), partial CSB-YP386076OFF1
CSB-EP386076OFF1
CSB-BP386076OFF1
CSB-MP386076OFF1
CSB-EP386076OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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