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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei 60 kDa chaperonin (groL), partial CSB-YP387058BPP
CSB-EP387058BPP
CSB-BP387058BPP
CSB-MP387058BPP
CSB-EP387058BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP387059BPP
CSB-EP387059BPP
CSB-BP387059BPP
CSB-MP387059BPP
CSB-EP387059BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP387060BPP
CSB-EP387060BPP
CSB-BP387060BPP
CSB-MP387060BPP
CSB-EP387060BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Elongation factor P (efp) CSB-YP387061BPP
CSB-EP387061BPP
CSB-BP387061BPP
CSB-MP387061BPP
CSB-EP387061BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dihydroxy-acid dehydratase (ilvD), partial CSB-YP387062BPP
CSB-EP387062BPP
CSB-BP387062BPP
CSB-MP387062BPP
CSB-EP387062BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Methionine--tRNA ligase (metG), partial CSB-YP387063BPP
CSB-EP387063BPP
CSB-BP387063BPP
CSB-MP387063BPP
CSB-EP387063BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein hscA homolog (hscA), partial CSB-YP387064BPP
CSB-EP387064BPP
CSB-BP387064BPP
CSB-MP387064BPP
CSB-EP387064BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Enolase (eno) CSB-YP387065BPP
CSB-EP387065BPP
CSB-BP387065BPP
CSB-MP387065BPP
CSB-EP387065BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glutamate--tRNA ligase (gltX) CSB-YP387066BPP
CSB-EP387066BPP
CSB-BP387066BPP
CSB-MP387066BPP
CSB-EP387066BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP387067BPP
CSB-EP387067BPP
CSB-BP387067BPP
CSB-MP387067BPP
CSB-EP387067BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP387068BPP
CSB-EP387068BPP
CSB-BP387068BPP
CSB-MP387068BPP
CSB-EP387068BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribonuclease HII (rnhB) CSB-YP387069BPP
CSB-EP387069BPP
CSB-BP387069BPP
CSB-MP387069BPP
CSB-EP387069BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP387070BPP
CSB-EP387070BPP
CSB-BP387070BPP
CSB-MP387070BPP
CSB-EP387070BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP387071BPP
CSB-EP387071BPP
CSB-BP387071BPP
CSB-MP387071BPP
CSB-EP387071BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Protein RecA (recA) CSB-YP387072MNX
CSB-EP387072MNX
CSB-BP387072MNX
CSB-MP387072MNX
CSB-EP387072MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Leucine--tRNA ligase (leuS), partial CSB-YP387073MNX
CSB-EP387073MNX
CSB-BP387073MNX
CSB-MP387073MNX
CSB-EP387073MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 7-cyano-7-deazaguanine synthase (queC) CSB-YP387074MNX
CSB-EP387074MNX
CSB-BP387074MNX
CSB-MP387074MNX
CSB-EP387074MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP387075MNX
CSB-EP387075MNX
CSB-BP387075MNX
CSB-MP387075MNX
CSB-EP387075MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 60 kDa chaperonin (groL), partial CSB-YP387076MNX
CSB-EP387076MNX
CSB-BP387076MNX
CSB-MP387076MNX
CSB-EP387076MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP387077MNX
CSB-EP387077MNX
CSB-BP387077MNX
CSB-MP387077MNX
CSB-EP387077MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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