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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methanoculleus marisnigri 30S ribosomal protein S12P (rps12p) CSB-YP386258MNU
CSB-EP386258MNU
CSB-BP386258MNU
CSB-MP386258MNU
CSB-EP386258MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0218 protein Memar_2244 (Memar_2244) CSB-YP386259MNU
CSB-EP386259MNU
CSB-BP386259MNU
CSB-MP386259MNU
CSB-EP386259MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0210 protein Memar_2269 (Memar_2269) CSB-YP386260MNU
CSB-EP386260MNU
CSB-BP386260MNU
CSB-MP386260MNU
CSB-EP386260MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA replication and repair protein recF (recF) CSB-YP386261STL
CSB-EP386261STL
CSB-BP386261STL
CSB-MP386261STL
CSB-EP386261STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP386262STL
CSB-EP386262STL
CSB-BP386262STL
CSB-MP386262STL
CSB-EP386262STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein smg homolog (smg) CSB-YP386263STL
CSB-EP386263STL
CSB-BP386263STL
CSB-MP386263STL
CSB-EP386263STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP386264STL
CSB-EP386264STL
CSB-BP386264STL
CSB-MP386264STL
CSB-EP386264STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Fe/S biogenesis protein NfuA (nfuA) CSB-YP386265STL
CSB-EP386265STL
CSB-BP386265STL
CSB-MP386265STL
CSB-EP386265STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP386266STL
CSB-EP386266STL
CSB-BP386266STL
CSB-MP386266STL
CSB-EP386266STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP386267STL
CSB-EP386267STL
CSB-BP386267STL
CSB-MP386267STL
CSB-EP386267STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0758 protein Sbal_0377 (Sbal_0377) CSB-YP386268STL
CSB-EP386268STL
CSB-BP386268STL
CSB-MP386268STL
CSB-EP386268STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP386269STL
CSB-EP386269STL
CSB-BP386269STL
CSB-MP386269STL
CSB-EP386269STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP386270STL
CSB-EP386270STL
CSB-BP386270STL
CSB-MP386270STL
CSB-EP386270STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP386271STL
CSB-EP386271STL
CSB-BP386271STL
CSB-MP386271STL
CSB-EP386271STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA mismatch repair protein mutL (mutL), partial CSB-YP386272STL
CSB-EP386272STL
CSB-BP386272STL
CSB-MP386272STL
CSB-EP386272STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP386273STL
CSB-EP386273STL
CSB-BP386273STL
CSB-MP386273STL
CSB-EP386273STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glutamyl-tRNA reductase (hemA) CSB-YP386274STL
CSB-EP386274STL
CSB-BP386274STL
CSB-MP386274STL
CSB-EP386274STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0114 protein Sbal_0780 (Sbal_0780), partial CSB-YP386275STL1
CSB-EP386275STL1
CSB-BP386275STL1
CSB-MP386275STL1
CSB-EP386275STL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP386276STL
CSB-EP386276STL
CSB-BP386276STL
CSB-MP386276STL
CSB-EP386276STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein slyX homolog (slyX) CSB-YP386277STL
CSB-EP386277STL
CSB-BP386277STL
CSB-MP386277STL
CSB-EP386277STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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