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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Scheffersomyces stipitis ATP-dependent rRNA helicase SPB4 (SPB4), partial CSB-YP387658SXM
CSB-EP387658SXM
CSB-BP387658SXM
CSB-MP387658SXM
CSB-EP387658SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis 40S ribosomal protein S1-B (RPS1B) CSB-YP387659SXM
CSB-EP387659SXM
CSB-BP387659SXM
CSB-MP387659SXM
CSB-EP387659SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Histone H2A.2 (HTA2) CSB-YP387660SXM
CSB-EP387660SXM
CSB-BP387660SXM
CSB-MP387660SXM
CSB-EP387660SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Nucleolar protein 9 (NOP9), partial CSB-YP387661SXM
CSB-EP387661SXM
CSB-BP387661SXM
CSB-MP387661SXM
CSB-EP387661SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis SWI5-dependent HO expression protein 3 (SHE3) CSB-YP387662SXM
CSB-EP387662SXM
CSB-BP387662SXM
CSB-MP387662SXM
CSB-EP387662SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP387663SXM
CSB-EP387663SXM
CSB-BP387663SXM
CSB-MP387663SXM
CSB-EP387663SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Long chronological lifespan protein 2 (LCL2) CSB-YP387664SXM
CSB-EP387664SXM
CSB-BP387664SXM
CSB-MP387664SXM
CSB-EP387664SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis DNA mismatch repair protein HSM3 (HSM3) CSB-YP387665SXM
CSB-EP387665SXM
CSB-BP387665SXM
CSB-MP387665SXM
CSB-EP387665SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Stress response protein NST1 (NST1), partial CSB-YP387666SXM
CSB-EP387666SXM
CSB-BP387666SXM
CSB-MP387666SXM
CSB-EP387666SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP387667SXM
CSB-EP387667SXM
CSB-BP387667SXM
CSB-MP387667SXM
CSB-EP387667SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Adenylosuccinate synthetase (PICST_85387) CSB-YP387668SXM
CSB-EP387668SXM
CSB-BP387668SXM
CSB-MP387668SXM
CSB-EP387668SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis COPII coat assembly protein SEC16 (SEC16), partial CSB-YP387669SXM
CSB-EP387669SXM
CSB-BP387669SXM
CSB-MP387669SXM
CSB-EP387669SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Altered inheritance of mitochondria protein 24, mitochondrial (AIM24) CSB-YP387670SXM
CSB-EP387670SXM
CSB-BP387670SXM
CSB-MP387670SXM
CSB-EP387670SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Actin cytoskeleton-regulatory complex protein END3 (END3) CSB-YP387671SXM
CSB-EP387671SXM
CSB-BP387671SXM
CSB-MP387671SXM
CSB-EP387671SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Lon protease homolog, mitochondrial (PIM1), partial CSB-YP387672SXM
CSB-EP387672SXM
CSB-BP387672SXM
CSB-MP387672SXM
CSB-EP387672SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Lipoprotein signal peptidase (lspA), partial CSB-YP387673AUD1
CSB-EP387673AUD1
CSB-BP387673AUD1
CSB-MP387673AUD1
CSB-EP387673AUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii ATP synthase subunit c (atpE), partial CSB-YP387674AUD1
CSB-EP387674AUD1
CSB-BP387674AUD1
CSB-MP387674AUD1
CSB-EP387674AUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Transcriptional repressor NrdR (nrdR) CSB-YP387675AUD
CSB-EP387675AUD
CSB-BP387675AUD
CSB-MP387675AUD
CSB-EP387675AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L1 (rplA) CSB-YP387676AUD
CSB-EP387676AUD
CSB-BP387676AUD
CSB-MP387676AUD
CSB-EP387676AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP387677AUD
CSB-EP387677AUD
CSB-BP387677AUD
CSB-MP387677AUD
CSB-EP387677AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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