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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Scheffersomyces stipitis Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP386498SXM
CSB-EP386498SXM
CSB-BP386498SXM
CSB-MP386498SXM
CSB-EP386498SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mitochondrial thiamine pyrophosphate carrier 1 (TPC1), partial CSB-YP386499SXM1
CSB-EP386499SXM1
CSB-BP386499SXM1
CSB-MP386499SXM1
CSB-EP386499SXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Putative tyrosine-protein phosphatase OCA1 (OCA1) CSB-YP386500SXM
CSB-EP386500SXM
CSB-BP386500SXM
CSB-MP386500SXM
CSB-EP386500SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mitochondrial distribution and morphology protein 12 (MDM12) CSB-YP386501SXM
CSB-EP386501SXM
CSB-BP386501SXM
CSB-MP386501SXM
CSB-EP386501SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis WD repeat-containing protein PICST_83842 (PRW1), partial CSB-YP386502SXM
CSB-EP386502SXM
CSB-BP386502SXM
CSB-MP386502SXM
CSB-EP386502SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Protein SEY1 (SEY1), partial CSB-YP386503SXM
CSB-EP386503SXM
CSB-BP386503SXM
CSB-MP386503SXM
CSB-EP386503SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ATP-dependent RNA helicase DHH1 (DHH1), partial CSB-YP386504SXM
CSB-EP386504SXM
CSB-BP386504SXM
CSB-MP386504SXM
CSB-EP386504SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Vacuolar protein sorting-associated protein 27 (VPS27), partial CSB-YP386505SXM
CSB-EP386505SXM
CSB-BP386505SXM
CSB-MP386505SXM
CSB-EP386505SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Pro-apoptotic serine protease NMA111 (NMA111), partial CSB-YP386506SXM
CSB-EP386506SXM
CSB-BP386506SXM
CSB-MP386506SXM
CSB-EP386506SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Histone H2B.2 (HTB2) CSB-YP386507SXM
CSB-EP386507SXM
CSB-BP386507SXM
CSB-MP386507SXM
CSB-EP386507SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis tRNA guanosine-2'-O-methyltransferase TRM13 (TRM13) CSB-YP386508SXM
CSB-EP386508SXM
CSB-BP386508SXM
CSB-MP386508SXM
CSB-EP386508SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Cytosolic Fe-S cluster assembly factor NAR1 (NAR1), partial CSB-YP386509SXM
CSB-EP386509SXM
CSB-BP386509SXM
CSB-MP386509SXM
CSB-EP386509SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis 54S ribosomal protein L4, mitochondrial (MRPL4), partial CSB-YP386510SXM
CSB-EP386510SXM
CSB-BP386510SXM
CSB-MP386510SXM
CSB-EP386510SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis ATP-dependent RNA helicase DBP10 (DBP10), partial CSB-YP386511SXM
CSB-EP386511SXM
CSB-BP386511SXM
CSB-MP386511SXM
CSB-EP386511SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Histone H2B.1 (HTB1) CSB-YP386512SXM
CSB-EP386512SXM
CSB-BP386512SXM
CSB-MP386512SXM
CSB-EP386512SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mitochondrial escape protein 2 (YME2), partial CSB-YP386513SXM
CSB-EP386513SXM
CSB-BP386513SXM
CSB-MP386513SXM
CSB-EP386513SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Potential protein lysine methyltransferase SET5 (SET5) CSB-YP386514SXM
CSB-EP386514SXM
CSB-BP386514SXM
CSB-MP386514SXM
CSB-EP386514SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Chromosomal replication initiator protein DnaA (dnaA) CSB-YP386515AUD
CSB-EP386515AUD
CSB-BP386515AUD
CSB-MP386515AUD
CSB-EP386515AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP386516AUD
CSB-EP386516AUD
CSB-BP386516AUD
CSB-MP386516AUD
CSB-EP386516AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii ATP synthase epsilon chain (atpC) CSB-YP386517AUD
CSB-EP386517AUD
CSB-BP386517AUD
CSB-MP386517AUD
CSB-EP386517AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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