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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 5b N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP387919AUF
CSB-EP387919AUF
CSB-BP387919AUF
CSB-MP387919AUF
CSB-EP387919AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Hydroxylamine reductase (hcp), partial CSB-YP387920AUF
CSB-EP387920AUF
CSB-BP387920AUF
CSB-MP387920AUF
CSB-EP387920AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 30S ribosomal protein S20 (rpsT) CSB-YP387921AUF
CSB-EP387921AUF
CSB-BP387921AUF
CSB-MP387921AUF
CSB-EP387921AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Octanoyltransferase (lipB) CSB-YP387922AUF
CSB-EP387922AUF
CSB-BP387922AUF
CSB-MP387922AUF
CSB-EP387922AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Urease accessory protein UreG (ureG) CSB-YP387923AUF
CSB-EP387923AUF
CSB-BP387923AUF
CSB-MP387923AUF
CSB-EP387923AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Urease subunit beta (ureB) CSB-YP387924AUF
CSB-EP387924AUF
CSB-BP387924AUF
CSB-MP387924AUF
CSB-EP387924AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP387925AUF
CSB-EP387925AUF
CSB-BP387925AUF
CSB-MP387925AUF
CSB-EP387925AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b D-ribose pyranase (rbsD) CSB-YP387926AUF
CSB-EP387926AUF
CSB-BP387926AUF
CSB-MP387926AUF
CSB-EP387926AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP387927AUF
CSB-EP387927AUF
CSB-BP387927AUF
CSB-MP387927AUF
CSB-EP387927AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP387928AUF
CSB-EP387928AUF
CSB-BP387928AUF
CSB-MP387928AUF
CSB-EP387928AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP387929AUF
CSB-EP387929AUF
CSB-BP387929AUF
CSB-MP387929AUF
CSB-EP387929AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Glucosamine-6-phosphate deaminase (nagB) CSB-YP387930AUF
CSB-EP387930AUF
CSB-BP387930AUF
CSB-MP387930AUF
CSB-EP387930AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L4 (rplD) CSB-YP387931AUF
CSB-EP387931AUF
CSB-BP387931AUF
CSB-MP387931AUF
CSB-EP387931AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L22 (rplV) CSB-YP387932AUF
CSB-EP387932AUF
CSB-BP387932AUF
CSB-MP387932AUF
CSB-EP387932AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 30S ribosomal protein S14 (rpsN) CSB-YP387933AUF
CSB-EP387933AUF
CSB-BP387933AUF
CSB-MP387933AUF
CSB-EP387933AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L30 (rpmD) CSB-YP387934AUF
CSB-EP387934AUF
CSB-BP387934AUF
CSB-MP387934AUF
CSB-EP387934AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L36 1 (rpmJ1) CSB-YP387935AUF
CSB-EP387935AUF
CSB-BP387935AUF
CSB-MP387935AUF
CSB-EP387935AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 30S ribosomal protein S4 (rpsD) CSB-YP387936AUF
CSB-EP387936AUF
CSB-BP387936AUF
CSB-MP387936AUF
CSB-EP387936AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b tRNA (guanine-N (1)-)-methyltransferase CSB-YP387937AUF
CSB-EP387937AUF
CSB-BP387937AUF
CSB-MP387937AUF
CSB-EP387937AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L31 type B (rpmE2) CSB-YP387938AUF
CSB-EP387938AUF
CSB-BP387938AUF
CSB-MP387938AUF
CSB-EP387938AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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