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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP388520RIJ
CSB-EP388520RIJ
CSB-BP388520RIJ
CSB-MP388520RIJ
CSB-EP388520RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable transcriptional regulatory protein Rsph17029_2308 (Rsph17029_2308) CSB-YP388521RIJ
CSB-EP388521RIJ
CSB-BP388521RIJ
CSB-MP388521RIJ
CSB-EP388521RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Formate--tetrahydrofolate ligase (fhs), partial CSB-YP388522RIJ
CSB-EP388522RIJ
CSB-BP388522RIJ
CSB-MP388522RIJ
CSB-EP388522RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Large-conductance mechanosensitive channel (mscL), partial CSB-YP388523RIJ1
CSB-EP388523RIJ1
CSB-BP388523RIJ1
CSB-MP388523RIJ1
CSB-EP388523RIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Pyrroloquinoline-quinone synthase (pqqC) CSB-YP388524RIJ
CSB-EP388524RIJ
CSB-BP388524RIJ
CSB-MP388524RIJ
CSB-EP388524RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP388525RIJ
CSB-EP388525RIJ
CSB-BP388525RIJ
CSB-MP388525RIJ
CSB-EP388525RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0178 protein Rsph17029_2512 (Rsph17029_2512) CSB-YP388526RIJ
CSB-EP388526RIJ
CSB-BP388526RIJ
CSB-MP388526RIJ
CSB-EP388526RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribonuclease H (rnhA) CSB-YP388527RIJ
CSB-EP388527RIJ
CSB-BP388527RIJ
CSB-MP388527RIJ
CSB-EP388527RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides SsrA-binding protein (smpB) CSB-YP388528RIJ
CSB-EP388528RIJ
CSB-BP388528RIJ
CSB-MP388528RIJ
CSB-EP388528RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Adenine deaminase (ade), partial CSB-YP388529RIJ
CSB-EP388529RIJ
CSB-BP388529RIJ
CSB-MP388529RIJ
CSB-EP388529RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L19 (rplS) CSB-YP388531RIJ
CSB-EP388531RIJ
CSB-BP388531RIJ
CSB-MP388531RIJ
CSB-EP388531RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L34 (rpmH) CSB-YP388532RIJ
CSB-EP388532RIJ
CSB-BP388532RIJ
CSB-MP388532RIJ
CSB-EP388532RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Acetylglutamate kinase (argB) CSB-YP388533RIJ
CSB-EP388533RIJ
CSB-BP388533RIJ
CSB-MP388533RIJ
CSB-EP388533RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S15 (rpsO) CSB-YP388534RIJ
CSB-EP388534RIJ
CSB-BP388534RIJ
CSB-MP388534RIJ
CSB-EP388534RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP388535RIJ
CSB-EP388535RIJ
CSB-BP388535RIJ
CSB-MP388535RIJ
CSB-EP388535RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Shikimate dehydrogenase (aroE) CSB-YP388536RIJ
CSB-EP388536RIJ
CSB-BP388536RIJ
CSB-MP388536RIJ
CSB-EP388536RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Gas vesicle structural protein (gvpA) CSB-YP388537RIJ
CSB-EP388537RIJ
CSB-BP388537RIJ
CSB-MP388537RIJ
CSB-EP388537RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP388538RIJ
CSB-EP388538RIJ
CSB-BP388538RIJ
CSB-MP388538RIJ
CSB-EP388538RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L21 (rplU) CSB-YP388539RIJ
CSB-EP388539RIJ
CSB-BP388539RIJ
CSB-MP388539RIJ
CSB-EP388539RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Dihydroxy-acid dehydratase (ilvD), partial CSB-YP388540RIJ
CSB-EP388540RIJ
CSB-BP388540RIJ
CSB-MP388540RIJ
CSB-EP388540RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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