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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis Phenylalanine--tRNA ligase alpha subunit (pheS), partial CSB-YP389382MNP
CSB-EP389382MNP
CSB-BP389382MNP
CSB-MP389382MNP
CSB-EP389382MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0278 protein MmarC5_0106 (MmarC5_0106) CSB-YP389383MNP
CSB-EP389383MNP
CSB-BP389383MNP
CSB-MP389383MNP
CSB-EP389383MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis GMP synthase [glutamine-hydrolyzing] subunit A (guaAA) CSB-YP389384MNP
CSB-EP389384MNP
CSB-BP389384MNP
CSB-MP389384MNP
CSB-EP389384MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2-phosphoglycerate kinase (pgk2) CSB-YP389385MNP
CSB-EP389385MNP
CSB-BP389385MNP
CSB-MP389385MNP
CSB-EP389385MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S5P (rps5p) CSB-YP389386MNP
CSB-EP389386MNP
CSB-BP389386MNP
CSB-MP389386MNP
CSB-EP389386MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S8P (rps8p) CSB-YP389387MNP
CSB-EP389387MNP
CSB-BP389387MNP
CSB-MP389387MNP
CSB-EP389387MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L5P (rpl5p) CSB-YP389388MNP
CSB-EP389388MNP
CSB-BP389388MNP
CSB-MP389388MNP
CSB-EP389388MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L29P (rpl29p) CSB-YP389389MNP
CSB-EP389389MNP
CSB-BP389389MNP
CSB-MP389389MNP
CSB-EP389389MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 3-dehydroquinate dehydratase (aroD) CSB-YP389390MNP
CSB-EP389390MNP
CSB-BP389390MNP
CSB-MP389390MNP
CSB-EP389390MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Elongation factor 1-alpha (tuf) CSB-YP389391MNP
CSB-EP389391MNP
CSB-BP389391MNP
CSB-MP389391MNP
CSB-EP389391MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA-directed RNA polymerase subunit H (rpoH) CSB-YP389392MNP
CSB-EP389392MNP
CSB-BP389392MNP
CSB-MP389392MNP
CSB-EP389392MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S11P (rps11p) CSB-YP389393MNP
CSB-EP389393MNP
CSB-BP389393MNP
CSB-MP389393MNP
CSB-EP389393MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0095 protein MmarC5_0307 (MmarC5_0307) CSB-YP389394MNP
CSB-EP389394MNP
CSB-BP389394MNP
CSB-MP389394MNP
CSB-EP389394MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP389395MNP
CSB-EP389395MNP
CSB-BP389395MNP
CSB-MP389395MNP
CSB-EP389395MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA repair and recombination protein radA (radA) CSB-YP389396MNP
CSB-EP389396MNP
CSB-BP389396MNP
CSB-MP389396MNP
CSB-EP389396MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Histidinol-phosphate aminotransferase (hisC) CSB-YP389397MNP
CSB-EP389397MNP
CSB-BP389397MNP
CSB-MP389397MNP
CSB-EP389397MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose-5-phosphate isomerase A (rpiA) CSB-YP389398MNP
CSB-EP389398MNP
CSB-BP389398MNP
CSB-MP389398MNP
CSB-EP389398MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Undecaprenyl-diphosphatase (uppP), partial CSB-YP389399MNP
CSB-EP389399MNP
CSB-BP389399MNP
CSB-MP389399MNP
CSB-EP389399MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Peptide chain release factor subunit 1 (prf1) CSB-YP389400MNP
CSB-EP389400MNP
CSB-BP389400MNP
CSB-MP389400MNP
CSB-EP389400MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoglucosamine mutase (glmM) CSB-YP389401MNP
CSB-EP389401MNP
CSB-BP389401MNP
CSB-MP389401MNP
CSB-EP389401MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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