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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L29 (rpmC) CSB-YP389362STB
CSB-EP389362STB
CSB-BP389362STB
CSB-MP389362STB
CSB-EP389362STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L2 (rplB) CSB-YP389363STB
CSB-EP389363STB
CSB-BP389363STB
CSB-MP389363STB
CSB-EP389363STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L4 (rplD) CSB-YP389364STB
CSB-EP389364STB
CSB-BP389364STB
CSB-MP389364STB
CSB-EP389364STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 30S ribosomal protein S12 (rpsL) CSB-YP389365STB
CSB-EP389365STB
CSB-BP389365STB
CSB-MP389365STB
CSB-EP389365STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L7/L12 (rplL) CSB-YP389366STB
CSB-EP389366STB
CSB-BP389366STB
CSB-MP389366STB
CSB-EP389366STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L11 (rplK) CSB-YP389367STB
CSB-EP389367STB
CSB-BP389367STB
CSB-MP389367STB
CSB-EP389367STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP389368STB
CSB-EP389368STB
CSB-BP389368STB
CSB-MP389368STB
CSB-EP389368STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP389369STB
CSB-EP389369STB
CSB-BP389369STB
CSB-MP389369STB
CSB-EP389369STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP389370STB
CSB-EP389370STB
CSB-BP389370STB
CSB-MP389370STB
CSB-EP389370STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP389371STB
CSB-EP389371STB
CSB-BP389371STB
CSB-MP389371STB
CSB-EP389371STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP389372STB
CSB-EP389372STB
CSB-BP389372STB
CSB-MP389372STB
CSB-EP389372STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L9 (rplI) CSB-YP389373STB
CSB-EP389373STB
CSB-BP389373STB
CSB-MP389373STB
CSB-EP389373STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L34 (rpmH) CSB-YP389374STB
CSB-EP389374STB
CSB-BP389374STB
CSB-MP389374STB
CSB-EP389374STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Phospholipase A2 natratoxin CSB-YP389375NFG
CSB-EP389375NFG
CSB-BP389375NFG
CSB-MP389375NFG
CSB-EP389375NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Lysosome-associated membrane glycoprotein 5 (LAMP5), partial CSB-YP389376BO
CSB-EP389376BO
CSB-BP389376BO
CSB-MP389376BO
CSB-EP389376BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA-binding protein 48 (rbm48) CSB-YP389377DIL
CSB-EP389377DIL
CSB-BP389377DIL
CSB-MP389377DIL
CSB-EP389377DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L15P (rpl15p) CSB-YP389378MNP
CSB-EP389378MNP
CSB-BP389378MNP
CSB-MP389378MNP
CSB-EP389378MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit H (mtrH) CSB-YP389379MNP
CSB-EP389379MNP
CSB-BP389379MNP
CSB-MP389379MNP
CSB-EP389379MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit E (mtrE), partial CSB-YP389380MNP1
CSB-EP389380MNP1
CSB-BP389380MNP1
CSB-MP389380MNP1
CSB-EP389380MNP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L4P (rpl4p) CSB-YP389381MNP
CSB-EP389381MNP
CSB-BP389381MNP
CSB-MP389381MNP
CSB-EP389381MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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