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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacillus thermodenitrificans Transcriptional regulator MntR (mntR) CSB-YP390182GEH
CSB-EP390182GEH
CSB-BP390182GEH
CSB-MP390182GEH
CSB-EP390182GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L33 2 (rpmG2) CSB-YP390183GEH
CSB-EP390183GEH
CSB-BP390183GEH
CSB-MP390183GEH
CSB-EP390183GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Putative phosphotransferase GTNG_2421 (GTNG_2421) CSB-YP390184GEH
CSB-EP390184GEH
CSB-BP390184GEH
CSB-MP390184GEH
CSB-EP390184GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ribosomal protein L11 methyltransferase (prmA) CSB-YP390185GEH
CSB-EP390185GEH
CSB-BP390185GEH
CSB-MP390185GEH
CSB-EP390185GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP390186GEH
CSB-EP390186GEH
CSB-BP390186GEH
CSB-MP390186GEH
CSB-EP390186GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Transcription elongation factor GreA (greA) CSB-YP390187GEH
CSB-EP390187GEH
CSB-BP390187GEH
CSB-MP390187GEH
CSB-EP390187GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Putative Holliday junction resolvase (GTNG_2487) CSB-YP390188GEH
CSB-EP390188GEH
CSB-BP390188GEH
CSB-MP390188GEH
CSB-EP390188GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Alanine--tRNA ligase (alaS), partial CSB-YP390189GEH
CSB-EP390189GEH
CSB-BP390189GEH
CSB-MP390189GEH
CSB-EP390189GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans D-tyrosyl-tRNA (Tyr) deacylase CSB-YP390190GEH
CSB-EP390190GEH
CSB-BP390190GEH
CSB-MP390190GEH
CSB-EP390190GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP390191GEH
CSB-EP390191GEH
CSB-BP390191GEH
CSB-MP390191GEH
CSB-EP390191GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans GTPase obg (obg) CSB-YP390192GEH
CSB-EP390192GEH
CSB-BP390192GEH
CSB-MP390192GEH
CSB-EP390192GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Trigger factor (tig) CSB-YP390193GEH
CSB-EP390193GEH
CSB-BP390193GEH
CSB-MP390193GEH
CSB-EP390193GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans MutS2 protein (mutS2), partial CSB-YP390194GEH
CSB-EP390194GEH
CSB-BP390194GEH
CSB-MP390194GEH
CSB-EP390194GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP390195GEH
CSB-EP390195GEH
CSB-BP390195GEH
CSB-MP390195GEH
CSB-EP390195GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0173 metal-dependent hydrolase GTNG_2675 (GTNG_2675) CSB-YP390196GEH
CSB-EP390196GEH
CSB-BP390196GEH
CSB-MP390196GEH
CSB-EP390196GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Septation ring formation regulator EzrA (ezrA), partial CSB-YP390197GEH
CSB-EP390197GEH
CSB-BP390197GEH
CSB-MP390197GEH
CSB-EP390197GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP390198GEH
CSB-EP390198GEH
CSB-BP390198GEH
CSB-MP390198GEH
CSB-EP390198GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP390199GEH
CSB-EP390199GEH
CSB-BP390199GEH
CSB-MP390199GEH
CSB-EP390199GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0457 protein GTNG_2792 (GTNG_2792) CSB-YP390200GEH
CSB-EP390200GEH
CSB-BP390200GEH
CSB-MP390200GEH
CSB-EP390200GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Small, acid-soluble spore protein H 3 (sspH3) CSB-YP390201GEH
CSB-EP390201GEH
CSB-BP390201GEH
CSB-MP390201GEH
CSB-EP390201GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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