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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aeromonas salmonicida DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP390782AUI
CSB-EP390782AUI
CSB-BP390782AUI
CSB-MP390782AUI
CSB-EP390782AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0265 protein ASA_0299 (ASA_0299) CSB-YP390783AUI
CSB-EP390783AUI
CSB-BP390783AUI
CSB-MP390783AUI
CSB-EP390783AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida UPF0042 nucleotide-binding protein ASA_0318 (ASA_0318) CSB-YP390784AUI
CSB-EP390784AUI
CSB-BP390784AUI
CSB-MP390784AUI
CSB-EP390784AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP390785AUI
CSB-EP390785AUI
CSB-BP390785AUI
CSB-MP390785AUI
CSB-EP390785AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Protein translocase subunit SecA (secA), partial CSB-YP390786AUI
CSB-EP390786AUI
CSB-BP390786AUI
CSB-MP390786AUI
CSB-EP390786AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP390787AUI
CSB-EP390787AUI
CSB-BP390787AUI
CSB-MP390787AUI
CSB-EP390787AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Bifunctional protein HldE (hldE) CSB-YP390788AUI
CSB-EP390788AUI
CSB-BP390788AUI
CSB-MP390788AUI
CSB-EP390788AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP390789AUI
CSB-EP390789AUI
CSB-BP390789AUI
CSB-MP390789AUI
CSB-EP390789AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 30S ribosomal protein S16 (rpsP) CSB-YP390790AUI
CSB-EP390790AUI
CSB-BP390790AUI
CSB-MP390790AUI
CSB-EP390790AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida RNA pyrophosphohydrolase (rppH) CSB-YP390791AUI
CSB-EP390791AUI
CSB-BP390791AUI
CSB-MP390791AUI
CSB-EP390791AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Isoleucine--tRNA ligase (ileS), partial CSB-YP390792AUI
CSB-EP390792AUI
CSB-BP390792AUI
CSB-MP390792AUI
CSB-EP390792AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 30S ribosomal protein S18 (rpsR) CSB-YP390793AUI
CSB-EP390793AUI
CSB-BP390793AUI
CSB-MP390793AUI
CSB-EP390793AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Protein TolB (tolB) CSB-YP390794AUI
CSB-EP390794AUI
CSB-BP390794AUI
CSB-MP390794AUI
CSB-EP390794AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP390795AUI
CSB-EP390795AUI
CSB-BP390795AUI
CSB-MP390795AUI
CSB-EP390795AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida DNA-binding protein fis (fis) CSB-YP390796AUI
CSB-EP390796AUI
CSB-BP390796AUI
CSB-MP390796AUI
CSB-EP390796AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Putative transport protein ASA_0825 (ASA_0825), partial CSB-YP390797AUI
CSB-EP390797AUI
CSB-BP390797AUI
CSB-MP390797AUI
CSB-EP390797AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Lysine--tRNA ligase (lysS), partial CSB-YP390798AUI
CSB-EP390798AUI
CSB-BP390798AUI
CSB-MP390798AUI
CSB-EP390798AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Chaperone protein TorD (torD) CSB-YP390799AUI
CSB-EP390799AUI
CSB-BP390799AUI
CSB-MP390799AUI
CSB-EP390799AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP390800AUI
CSB-EP390800AUI
CSB-BP390800AUI
CSB-MP390800AUI
CSB-EP390800AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Phosphoglucosamine mutase (glmM) CSB-YP390801AUI
CSB-EP390801AUI
CSB-BP390801AUI
CSB-MP390801AUI
CSB-EP390801AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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