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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis 30S ribosomal protein S9P (rps9p) CSB-YP389462MNP
CSB-EP389462MNP
CSB-BP389462MNP
CSB-MP389462MNP
CSB-EP389462MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis IMP cyclohydrolase (purO) CSB-YP389463MNP
CSB-EP389463MNP
CSB-BP389463MNP
CSB-MP389463MNP
CSB-EP389463MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0200 protein MmarC5_0311 (MmarC5_0311) CSB-YP389464MNP
CSB-EP389464MNP
CSB-BP389464MNP
CSB-MP389464MNP
CSB-EP389464MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis O-phospho-L-seryl-tRNA:Cys-tRNA synthase (MmarC5_0351) CSB-YP389465MNP
CSB-EP389465MNP
CSB-BP389465MNP
CSB-MP389465MNP
CSB-EP389465MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0219 protein MmarC5_0379 (MmarC5_0379) CSB-YP389466MNP
CSB-EP389466MNP
CSB-BP389466MNP
CSB-MP389466MNP
CSB-EP389466MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP389467MNP
CSB-EP389467MNP
CSB-BP389467MNP
CSB-MP389467MNP
CSB-EP389467MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis L-fuculose phosphate aldolase (fucA) CSB-YP389468MNP
CSB-EP389468MNP
CSB-BP389468MNP
CSB-MP389468MNP
CSB-EP389468MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Hydroxyethylthiazole kinase (thiM) CSB-YP389469MNP
CSB-EP389469MNP
CSB-BP389469MNP
CSB-MP389469MNP
CSB-EP389469MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0235 protein MmarC5_0538 (MmarC5_0538) CSB-YP389470MNP
CSB-EP389470MNP
CSB-BP389470MNP
CSB-MP389470MNP
CSB-EP389470MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase subunit F (atpF) CSB-YP389471MNP
CSB-EP389471MNP
CSB-BP389471MNP
CSB-MP389471MNP
CSB-EP389471MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Adenylate kinase (adkA) CSB-YP389472MNP
CSB-EP389472MNP
CSB-BP389472MNP
CSB-MP389472MNP
CSB-EP389472MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP389473MNP
CSB-EP389473MNP
CSB-BP389473MNP
CSB-MP389473MNP
CSB-EP389473MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Type-2 serine--tRNA ligase (serS), partial CSB-YP389474MNP
CSB-EP389474MNP
CSB-BP389474MNP
CSB-MP389474MNP
CSB-EP389474MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease P protein component 4 (rnp4) CSB-YP389475MNP
CSB-EP389475MNP
CSB-BP389475MNP
CSB-MP389475MNP
CSB-EP389475MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP389476MNP
CSB-EP389476MNP
CSB-BP389476MNP
CSB-MP389476MNP
CSB-EP389476MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis ATP phosphoribosyltransferase (hisG) CSB-YP389477MNP
CSB-EP389477MNP
CSB-BP389477MNP
CSB-MP389477MNP
CSB-EP389477MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 5A (eIF5A) CSB-YP389478MNP
CSB-EP389478MNP
CSB-BP389478MNP
CSB-MP389478MNP
CSB-EP389478MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable L-aspartate dehydrogenase (nadX) CSB-YP389479MNP
CSB-EP389479MNP
CSB-BP389479MNP
CSB-MP389479MNP
CSB-EP389479MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Proline--tRNA ligase (proS) CSB-YP389480MNP
CSB-EP389480MNP
CSB-BP389480MNP
CSB-MP389480MNP
CSB-EP389480MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Uracil phosphoribosyltransferase (upp) CSB-YP389481MNP
CSB-EP389481MNP
CSB-BP389481MNP
CSB-MP389481MNP
CSB-EP389481MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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