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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis UPF0305 protein MmarC5_0909 (MmarC5_0909) CSB-YP389482MNP
CSB-EP389482MNP
CSB-BP389482MNP
CSB-MP389482MNP
CSB-EP389482MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S28e (rps28e) CSB-YP389483MNP
CSB-EP389483MNP
CSB-BP389483MNP
CSB-MP389483MNP
CSB-EP389483MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L14e (rpl14e) CSB-YP389484MNP
CSB-EP389484MNP
CSB-BP389484MNP
CSB-MP389484MNP
CSB-EP389484MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA), partial CSB-YP389485MNP
CSB-EP389485MNP
CSB-BP389485MNP
CSB-MP389485MNP
CSB-EP389485MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) CSB-YP389486MNP
CSB-EP389486MNP
CSB-BP389486MNP
CSB-MP389486MNP
CSB-EP389486MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable molybdenum cofactor biosynthesis protein A (moaA) CSB-YP389487MNP
CSB-EP389487MNP
CSB-BP389487MNP
CSB-MP389487MNP
CSB-EP389487MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP389488MNP
CSB-EP389488MNP
CSB-BP389488MNP
CSB-MP389488MNP
CSB-EP389488MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Replication factor C small subunit (rfcS) CSB-YP389489MNP
CSB-EP389489MNP
CSB-BP389489MNP
CSB-MP389489MNP
CSB-EP389489MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis GTP cyclohydrolase III (gch3) CSB-YP389490MNP
CSB-EP389490MNP
CSB-BP389490MNP
CSB-MP389490MNP
CSB-EP389490MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Digeranylgeranylglycerophospholipid reductase (MmarC5_1250) CSB-YP389491MNP
CSB-EP389491MNP
CSB-BP389491MNP
CSB-MP389491MNP
CSB-EP389491MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative thymidine phosphorylase (MmarC5_1346), partial CSB-YP389492MNP
CSB-EP389492MNP
CSB-BP389492MNP
CSB-MP389492MNP
CSB-EP389492MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Replication factor C large subunit (rfcL) CSB-YP389493MNP
CSB-EP389493MNP
CSB-BP389493MNP
CSB-MP389493MNP
CSB-EP389493MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L15e (rpl15e) CSB-YP389494MNP
CSB-EP389494MNP
CSB-BP389494MNP
CSB-MP389494MNP
CSB-EP389494MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0248 protein MmarC5_1387 (MmarC5_1387) CSB-YP389495MNP
CSB-EP389495MNP
CSB-BP389495MNP
CSB-MP389495MNP
CSB-EP389495MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L1P (rpl1p) CSB-YP389496MNP
CSB-EP389496MNP
CSB-BP389496MNP
CSB-MP389496MNP
CSB-EP389496MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Prefoldin subunit beta (pfdB) CSB-YP389497MNP
CSB-EP389497MNP
CSB-BP389497MNP
CSB-MP389497MNP
CSB-EP389497MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine synthase (queC) CSB-YP389498MNP
CSB-EP389498MNP
CSB-BP389498MNP
CSB-MP389498MNP
CSB-EP389498MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphomethylpyrimidine synthase (thiC) CSB-YP389499MNP
CSB-EP389499MNP
CSB-BP389499MNP
CSB-MP389499MNP
CSB-EP389499MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L40e (rpl40e) CSB-YP389500MNP
CSB-EP389500MNP
CSB-BP389500MNP
CSB-MP389500MNP
CSB-EP389500MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5,10-methenyltetrahydromethanopterin hydrogenase (hmd) CSB-YP389501MNP
CSB-EP389501MNP
CSB-BP389501MNP
CSB-MP389501MNP
CSB-EP389501MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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