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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant FMN-dependent NADH-azoreductase (azoR) CSB-YP389522HSJ
CSB-EP389522HSJ
CSB-BP389522HSJ
CSB-MP389522HSJ
CSB-EP389522HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome maturation factor RimM (rimM) CSB-YP389523HSJ
CSB-EP389523HSJ
CSB-BP389523HSJ
CSB-MP389523HSJ
CSB-EP389523HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP389524HSJ
CSB-EP389524HSJ
CSB-BP389524HSJ
CSB-MP389524HSJ
CSB-EP389524HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein FolD (folD) CSB-YP389525HSJ
CSB-EP389525HSJ
CSB-BP389525HSJ
CSB-MP389525HSJ
CSB-EP389525HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0345 protein HEAR0870 (HEAR0870) CSB-YP389526HSJ
CSB-EP389526HSJ
CSB-BP389526HSJ
CSB-MP389526HSJ
CSB-EP389526HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP389527HSJ
CSB-EP389527HSJ
CSB-BP389527HSJ
CSB-MP389527HSJ
CSB-EP389527HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate--tRNA ligase (gltX) CSB-YP389528HSJ
CSB-EP389528HSJ
CSB-BP389528HSJ
CSB-MP389528HSJ
CSB-EP389528HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein HEAR1046 (HEAR1046) CSB-YP389529HSJ
CSB-EP389529HSJ
CSB-BP389529HSJ
CSB-MP389529HSJ
CSB-EP389529HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-5-phosphate isomerase A (rpiA) CSB-YP389530HSJ
CSB-EP389530HSJ
CSB-BP389530HSJ
CSB-MP389530HSJ
CSB-EP389530HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP389531HSJ
CSB-EP389531HSJ
CSB-BP389531HSJ
CSB-MP389531HSJ
CSB-EP389531HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP389532HSJ
CSB-EP389532HSJ
CSB-BP389532HSJ
CSB-MP389532HSJ
CSB-EP389532HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase Der (der) CSB-YP389533HSJ
CSB-EP389533HSJ
CSB-BP389533HSJ
CSB-MP389533HSJ
CSB-EP389533HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0502 protein HEAR1280 (HEAR1280) CSB-YP389534HSJ
CSB-EP389534HSJ
CSB-BP389534HSJ
CSB-MP389534HSJ
CSB-EP389534HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP389535HSJ
CSB-EP389535HSJ
CSB-BP389535HSJ
CSB-MP389535HSJ
CSB-EP389535HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridylate kinase (pyrH) CSB-YP389536HSJ
CSB-EP389536HSJ
CSB-BP389536HSJ
CSB-MP389536HSJ
CSB-EP389536HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP389537HSJ
CSB-EP389537HSJ
CSB-BP389537HSJ
CSB-MP389537HSJ
CSB-EP389537HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative phosphotransferase HEAR1351 (HEAR1351) CSB-YP389538HSJ
CSB-EP389538HSJ
CSB-BP389538HSJ
CSB-MP389538HSJ
CSB-EP389538HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potassium-transporting ATPase A chain (kdpA), partial CSB-YP389539HSJ
CSB-EP389539HSJ
CSB-BP389539HSJ
CSB-MP389539HSJ
CSB-EP389539HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP389540HSJ
CSB-EP389540HSJ
CSB-BP389540HSJ
CSB-MP389540HSJ
CSB-EP389540HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP389541HSJ
CSB-EP389541HSJ
CSB-BP389541HSJ
CSB-MP389541HSJ
CSB-EP389541HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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