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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacillus thermodenitrificans 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP391082GEH
CSB-EP391082GEH
CSB-BP391082GEH
CSB-MP391082GEH
CSB-EP391082GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Acireductone dioxygenase (mtnD) CSB-YP391083GEH
CSB-EP391083GEH
CSB-BP391083GEH
CSB-MP391083GEH
CSB-EP391083GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0358 protein GTNG_0942 (GTNG_0942) CSB-YP391084GEH
CSB-EP391084GEH
CSB-BP391084GEH
CSB-MP391084GEH
CSB-EP391084GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0298 protein GTNG_0961 (GTNG_0961) CSB-YP391085GEH
CSB-EP391085GEH
CSB-BP391085GEH
CSB-MP391085GEH
CSB-EP391085GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP391086GEH
CSB-EP391086GEH
CSB-BP391086GEH
CSB-MP391086GEH
CSB-EP391086GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Cell division protein SepF (sepF) CSB-YP391087GEH
CSB-EP391087GEH
CSB-BP391087GEH
CSB-MP391087GEH
CSB-EP391087GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Aspartate carbamoyltransferase (pyrB) CSB-YP391088GEH
CSB-EP391088GEH
CSB-BP391088GEH
CSB-MP391088GEH
CSB-EP391088GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Dihydroorotase (pyrC) CSB-YP391089GEH
CSB-EP391089GEH
CSB-BP391089GEH
CSB-MP391089GEH
CSB-EP391089GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0296 protein GTNG_1019 (GTNG_1019) CSB-YP391090GEH
CSB-EP391090GEH
CSB-BP391090GEH
CSB-MP391090GEH
CSB-EP391090GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Transcription factor fapR (fapR) CSB-YP391091GEH
CSB-EP391091GEH
CSB-BP391091GEH
CSB-MP391091GEH
CSB-EP391091GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ribosome maturation factor RimM (rimM) CSB-YP391092GEH
CSB-EP391092GEH
CSB-BP391092GEH
CSB-MP391092GEH
CSB-EP391092GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP391093GEH
CSB-EP391093GEH
CSB-BP391093GEH
CSB-MP391093GEH
CSB-EP391093GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Uridylate kinase (pyrH) CSB-YP391094GEH
CSB-EP391094GEH
CSB-BP391094GEH
CSB-MP391094GEH
CSB-EP391094GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP391095GEH
CSB-EP391095GEH
CSB-BP391095GEH
CSB-MP391095GEH
CSB-EP391095GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Small, acid-soluble spore protein P (sspP) CSB-YP391096GEH
CSB-EP391096GEH
CSB-BP391096GEH
CSB-MP391096GEH
CSB-EP391096GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Formimidoylglutamase (hutG) CSB-YP391097GEH
CSB-EP391097GEH
CSB-BP391097GEH
CSB-MP391097GEH
CSB-EP391097GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0145 protein GTNG_1265 (GTNG_1265) CSB-YP391098GEH
CSB-EP391098GEH
CSB-BP391098GEH
CSB-MP391098GEH
CSB-EP391098GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Small, acid-soluble spore protein H 1 (sspH1) CSB-YP391099GEH
CSB-EP391099GEH
CSB-BP391099GEH
CSB-MP391099GEH
CSB-EP391099GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Xanthine phosphoribosyltransferase (xpt) CSB-YP391100GEH
CSB-EP391100GEH
CSB-BP391100GEH
CSB-MP391100GEH
CSB-EP391100GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP391101GEH
CSB-EP391101GEH
CSB-BP391101GEH
CSB-MP391101GEH
CSB-EP391101GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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