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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP389542HSJ
CSB-EP389542HSJ
CSB-BP389542HSJ
CSB-MP389542HSJ
CSB-EP389542HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S15 (rpsO) CSB-YP389543HSJ
CSB-EP389543HSJ
CSB-BP389543HSJ
CSB-MP389543HSJ
CSB-EP389543HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP389544HSJ
CSB-EP389544HSJ
CSB-BP389544HSJ
CSB-MP389544HSJ
CSB-EP389544HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate acyltransferase (plsX) CSB-YP389545HSJ
CSB-EP389545HSJ
CSB-BP389545HSJ
CSB-MP389545HSJ
CSB-EP389545HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L9 (rplI) CSB-YP389546HSJ
CSB-EP389546HSJ
CSB-BP389546HSJ
CSB-MP389546HSJ
CSB-EP389546HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate synthase (dapA) CSB-YP389547HSJ
CSB-EP389547HSJ
CSB-BP389547HSJ
CSB-MP389547HSJ
CSB-EP389547HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysine--tRNA ligase (lysS), partial CSB-YP389548HSJ
CSB-EP389548HSJ
CSB-BP389548HSJ
CSB-MP389548HSJ
CSB-EP389548HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-binding factor A (rbfA) CSB-YP389549HSJ
CSB-EP389549HSJ
CSB-BP389549HSJ
CSB-MP389549HSJ
CSB-EP389549HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroorotate dehydrogenase (quinone) CSB-YP389550HSJ
CSB-EP389550HSJ
CSB-BP389550HSJ
CSB-MP389550HSJ
CSB-EP389550HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 (rpmG) CSB-YP389551HSJ
CSB-EP389551HSJ
CSB-BP389551HSJ
CSB-MP389551HSJ
CSB-EP389551HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP389552HSJ
CSB-EP389552HSJ
CSB-BP389552HSJ
CSB-MP389552HSJ
CSB-EP389552HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP389553HSJ
CSB-EP389553HSJ
CSB-BP389553HSJ
CSB-MP389553HSJ
CSB-EP389553HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP389554HSJ
CSB-EP389554HSJ
CSB-BP389554HSJ
CSB-MP389554HSJ
CSB-EP389554HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-glutamyl phosphate reductase (proA) CSB-YP389555HSJ
CSB-EP389555HSJ
CSB-BP389555HSJ
CSB-MP389555HSJ
CSB-EP389555HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP389556HSJ
CSB-EP389556HSJ
CSB-BP389556HSJ
CSB-MP389556HSJ
CSB-EP389556HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal protein L11 methyltransferase (prmA) CSB-YP389557HSJ
CSB-EP389557HSJ
CSB-BP389557HSJ
CSB-MP389557HSJ
CSB-EP389557HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate 5-kinase (proB) CSB-YP389558HSJ
CSB-EP389558HSJ
CSB-BP389558HSJ
CSB-MP389558HSJ
CSB-EP389558HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L27 (rpmA) CSB-YP389559HSJ
CSB-EP389559HSJ
CSB-BP389559HSJ
CSB-MP389559HSJ
CSB-EP389559HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP389560HSJ
CSB-EP389560HSJ
CSB-BP389560HSJ
CSB-MP389560HSJ
CSB-EP389560HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0042 nucleotide-binding protein HEAR2885 (HEAR2885) CSB-YP389561HSJ
CSB-EP389561HSJ
CSB-BP389561HSJ
CSB-MP389561HSJ
CSB-EP389561HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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