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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Populus trichocarpa Photosystem I reaction center subunit IX (psaJ), partial CSB-YP389622EXS
CSB-EP389622EXS
CSB-BP389622EXS
CSB-MP389622EXS
CSB-EP389622EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 30S ribosomal protein S12-A, chloroplastic (rps12-A) CSB-YP389623EXS
CSB-EP389623EXS
CSB-BP389623EXS
CSB-MP389623EXS
CSB-EP389623EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP389624EXS1
CSB-EP389624EXS1
CSB-BP389624EXS1
CSB-MP389624EXS1
CSB-EP389624EXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP389625EXS
CSB-EP389625EXS
CSB-BP389625EXS
CSB-MP389625EXS
CSB-EP389625EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP389626EXS
CSB-EP389626EXS
CSB-BP389626EXS
CSB-MP389626EXS
CSB-EP389626EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP389627EXS
CSB-EP389627EXS
CSB-BP389627EXS
CSB-MP389627EXS
CSB-EP389627EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP389628EXS
CSB-EP389628EXS
CSB-BP389628EXS
CSB-MP389628EXS
CSB-EP389628EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic, partial CSB-YP389629EXS
CSB-EP389629EXS
CSB-BP389629EXS
CSB-MP389629EXS
CSB-EP389629EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa NAD (P)H-quinone oxidoreductase subunit 1, chloroplastic, partial CSB-YP389630EXS1
CSB-EP389630EXS1
CSB-BP389630EXS1
CSB-MP389630EXS1
CSB-EP389630EXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torque teno midi virus 1 Uncharacterized ORF3 protein (ORF3) CSB-YP389631TKI
CSB-EP389631TKI
CSB-BP389631TKI
CSB-MP389631TKI
CSB-EP389631TKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Beta-defensin 104A (DEFB104A) CSB-YP389632GGZ
CSB-EP389632GGZ
CSB-BP389632GGZ
CSB-MP389632GGZ
CSB-EP389632GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Beta-defensin 105A (DEFB105A) CSB-YP389633GGZ
CSB-EP389633GGZ
CSB-BP389633GGZ
CSB-MP389633GGZ
CSB-EP389633GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Beta-defensin 105A (DEFB105A) CSB-YP389634MOV
CSB-EP389634MOV
CSB-BP389634MOV
CSB-MP389634MOV
CSB-EP389634MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Beta-defensin 107A (DEFB107A) CSB-YP389635EXP
CSB-EP389635EXP
CSB-BP389635EXP
CSB-MP389635EXP
CSB-EP389635EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0254 protein MmarC5_0742 (MmarC5_0742) CSB-YP389636MNP
CSB-EP389636MNP
CSB-BP389636MNP
CSB-MP389636MNP
CSB-EP389636MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP389637MNP
CSB-EP389637MNP
CSB-BP389637MNP
CSB-MP389637MNP
CSB-EP389637MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobalamin biosynthesis protein CobD (cobD), partial CSB-YP389638MNP1
CSB-EP389638MNP1
CSB-BP389638MNP1
CSB-MP389638MNP1
CSB-EP389638MNP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP389639MNP
CSB-EP389639MNP
CSB-BP389639MNP
CSB-MP389639MNP
CSB-EP389639MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UDP-N-acetyl-D-mannosamine dehydrogenase (wecC) CSB-YP389640MNP
CSB-EP389640MNP
CSB-BP389640MNP
CSB-MP389640MNP
CSB-EP389640MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis O-phosphoserine--tRNA (Cys) ligase, partial CSB-YP389641MNP
CSB-EP389641MNP
CSB-BP389641MNP
CSB-MP389641MNP
CSB-EP389641MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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