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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP389702HSJ
CSB-EP389702HSJ
CSB-BP389702HSJ
CSB-MP389702HSJ
CSB-EP389702HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LexA repressor (lexA) CSB-YP389703HSJ
CSB-EP389703HSJ
CSB-BP389703HSJ
CSB-MP389703HSJ
CSB-EP389703HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protease HtpX homolog (htpX), partial CSB-YP389704HSJ1
CSB-EP389704HSJ1
CSB-BP389704HSJ1
CSB-MP389704HSJ1
CSB-EP389704HSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease H (rnhA) CSB-YP389705HSJ
CSB-EP389705HSJ
CSB-BP389705HSJ
CSB-MP389705HSJ
CSB-EP389705HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative arginyl-tRNA--protein transferase (ate) CSB-YP389706HSJ
CSB-EP389706HSJ
CSB-BP389706HSJ
CSB-MP389706HSJ
CSB-EP389706HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0758 protein HEAR2468 (HEAR2468) CSB-YP389707HSJ
CSB-EP389707HSJ
CSB-BP389707HSJ
CSB-MP389707HSJ
CSB-EP389707HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP389708HSJ
CSB-EP389708HSJ
CSB-BP389708HSJ
CSB-MP389708HSJ
CSB-EP389708HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carboxylate-amine ligase HEAR2518 (HEAR2518) CSB-YP389709HSJ
CSB-EP389709HSJ
CSB-BP389709HSJ
CSB-MP389709HSJ
CSB-EP389709HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integration host factor subunit beta (ihfB) CSB-YP389710HSJ
CSB-EP389710HSJ
CSB-BP389710HSJ
CSB-MP389710HSJ
CSB-EP389710HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein recX (recX) CSB-YP389711HSJ
CSB-EP389711HSJ
CSB-BP389711HSJ
CSB-MP389711HSJ
CSB-EP389711HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP389712HSJ
CSB-EP389712HSJ
CSB-BP389712HSJ
CSB-MP389712HSJ
CSB-EP389712HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP389713HSJ
CSB-EP389713HSJ
CSB-BP389713HSJ
CSB-MP389713HSJ
CSB-EP389713HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP389714HSJ
CSB-EP389714HSJ
CSB-BP389714HSJ
CSB-MP389714HSJ
CSB-EP389714HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP389715HSJ
CSB-EP389715HSJ
CSB-BP389715HSJ
CSB-MP389715HSJ
CSB-EP389715HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proline--tRNA ligase (proS), partial CSB-YP389716HSJ
CSB-EP389716HSJ
CSB-BP389716HSJ
CSB-MP389716HSJ
CSB-EP389716HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase obg (obg) CSB-YP389717HSJ
CSB-EP389717HSJ
CSB-BP389717HSJ
CSB-MP389717HSJ
CSB-EP389717HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP389718HSJ
CSB-EP389718HSJ
CSB-BP389718HSJ
CSB-MP389718HSJ
CSB-EP389718HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein MraZ (mraZ) CSB-YP389719HSJ
CSB-EP389719HSJ
CSB-BP389719HSJ
CSB-MP389719HSJ
CSB-EP389719HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP389720HSJ
CSB-EP389720HSJ
CSB-BP389720HSJ
CSB-MP389720HSJ
CSB-EP389720HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type III pantothenate kinase (coaX) CSB-YP389721HSJ
CSB-EP389721HSJ
CSB-BP389721HSJ
CSB-MP389721HSJ
CSB-EP389721HSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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