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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia vietnamiensis Flavoprotein wrbA (wrbA) CSB-YP390422BPV
CSB-EP390422BPV
CSB-BP390422BPV
CSB-MP390422BPV
CSB-EP390422BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 4-hydroxy-2-oxovalerate aldolase 2 (Bcep1808_7605) CSB-YP390423BPV
CSB-EP390423BPV
CSB-BP390423BPV
CSB-MP390423BPV
CSB-EP390423BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP390424ILO
CSB-EP390424ILO
CSB-BP390424ILO
CSB-MP390424ILO
CSB-EP390424ILO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat C-type lectin domain family 2 member D6 (Clec2d6), partial CSB-YP390425RA
CSB-EP390425RA
CSB-BP390425RA
CSB-MP390425RA
CSB-EP390425RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens ascovirus 3e Putative matrix metalloproteinase (ORF20) CSB-YP390426HSI
CSB-EP390426HSI
CSB-BP390426HSI
CSB-MP390426HSI
CSB-EP390426HSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens ascovirus 3e Putative structural protein ORF146 (ORF146), partial CSB-YP390427HSI
CSB-EP390427HSI
CSB-BP390427HSI
CSB-MP390427HSI
CSB-EP390427HSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein LFL1 (LFL1) CSB-YP390428OFG
CSB-EP390428OFG
CSB-BP390428OFG
CSB-MP390428OFG
CSB-EP390428OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice gall dwarf virus Protein P7 CSB-YP390429RMJ
CSB-EP390429RMJ
CSB-BP390429RMJ
CSB-MP390429RMJ
CSB-EP390429RMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Zinc metalloproteinase-disintegrin VAP2A CSB-YP390430DYC
CSB-EP390430DYC
CSB-BP390430DYC
CSB-MP390430DYC
CSB-EP390430DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago truncatula S-adenosylmethionine synthase (METK) CSB-YP390431MQP
CSB-EP390431MQP
CSB-BP390431MQP
CSB-MP390431MQP
CSB-EP390431MQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum DNA replication and repair protein recF (recF) CSB-YP390432CXG
CSB-EP390432CXG
CSB-BP390432CXG
CSB-MP390432CXG
CSB-EP390432CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Biotin synthase (bioB) CSB-YP390433CXG
CSB-EP390433CXG
CSB-BP390433CXG
CSB-MP390433CXG
CSB-EP390433CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Urease accessory protein UreE (ureE) CSB-YP390434CXG
CSB-EP390434CXG
CSB-BP390434CXG
CSB-MP390434CXG
CSB-EP390434CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Urease accessory protein UreG (ureG) CSB-YP390435CXG
CSB-EP390435CXG
CSB-BP390435CXG
CSB-MP390435CXG
CSB-EP390435CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Putative 3-methyladenine DNA glycosylase (cgR_0192) CSB-YP390436CXG
CSB-EP390436CXG
CSB-BP390436CXG
CSB-MP390436CXG
CSB-EP390436CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Aspartate 1-decarboxylase (panD) CSB-YP390437CXG
CSB-EP390437CXG
CSB-BP390437CXG
CSB-MP390437CXG
CSB-EP390437CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP390438CXG
CSB-EP390438CXG
CSB-BP390438CXG
CSB-MP390438CXG
CSB-EP390438CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glutamyl-tRNA reductase (hemA) CSB-YP390439CXG
CSB-EP390439CXG
CSB-BP390439CXG
CSB-MP390439CXG
CSB-EP390439CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Quinate/shikimate dehydrogenase (aroE) CSB-YP390440CXG
CSB-EP390440CXG
CSB-BP390440CXG
CSB-MP390440CXG
CSB-EP390440CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Probable 5-dehydro-4-deoxyglucarate dehydratase (cgR_0526) CSB-YP390441CXG
CSB-EP390441CXG
CSB-BP390441CXG
CSB-MP390441CXG
CSB-EP390441CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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