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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. tularensis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP390222FDX
CSB-EP390222FDX
CSB-BP390222FDX
CSB-MP390222FDX
CSB-EP390222FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis tRNA (guanine-N (1)-)-methyltransferase CSB-YP390223FDX
CSB-EP390223FDX
CSB-BP390223FDX
CSB-MP390223FDX
CSB-EP390223FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Glutamyl-tRNA reductase (hemA) CSB-YP390224FDX
CSB-EP390224FDX
CSB-BP390224FDX
CSB-MP390224FDX
CSB-EP390224FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Cell division topological specificity factor (minE) CSB-YP390225FDX
CSB-EP390225FDX
CSB-BP390225FDX
CSB-MP390225FDX
CSB-EP390225FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 50S ribosomal protein L28 (rpmB) CSB-YP390226FDX
CSB-EP390226FDX
CSB-BP390226FDX
CSB-MP390226FDX
CSB-EP390226FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Lipid-A-disaccharide synthase (lpxB) CSB-YP390227FDX
CSB-EP390227FDX
CSB-BP390227FDX
CSB-MP390227FDX
CSB-EP390227FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP390228FDX
CSB-EP390228FDX
CSB-BP390228FDX
CSB-MP390228FDX
CSB-EP390228FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Proline--tRNA ligase (proS), partial CSB-YP390229FDX
CSB-EP390229FDX
CSB-BP390229FDX
CSB-MP390229FDX
CSB-EP390229FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 50S ribosomal protein L32 (rpmF) CSB-YP390230FDX
CSB-EP390230FDX
CSB-BP390230FDX
CSB-MP390230FDX
CSB-EP390230FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ribonuclease HII (rnhB) CSB-YP390231FDX
CSB-EP390231FDX
CSB-BP390231FDX
CSB-MP390231FDX
CSB-EP390231FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Protein grpE (grpE) CSB-YP390232FDX
CSB-EP390232FDX
CSB-BP390232FDX
CSB-MP390232FDX
CSB-EP390232FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis UPF0133 protein FTW_0607 (FTW_0607) CSB-YP390233FDX
CSB-EP390233FDX
CSB-BP390233FDX
CSB-MP390233FDX
CSB-EP390233FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP390234FDX
CSB-EP390234FDX
CSB-BP390234FDX
CSB-MP390234FDX
CSB-EP390234FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP390235FDX
CSB-EP390235FDX
CSB-BP390235FDX
CSB-MP390235FDX
CSB-EP390235FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP390236FDX
CSB-EP390236FDX
CSB-BP390236FDX
CSB-MP390236FDX
CSB-EP390236FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP390237FDX
CSB-EP390237FDX
CSB-BP390237FDX
CSB-MP390237FDX
CSB-EP390237FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis UPF0301 protein FTW_0891 (FTW_0891) CSB-YP390238FDX
CSB-EP390238FDX
CSB-BP390238FDX
CSB-MP390238FDX
CSB-EP390238FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP390239FDX
CSB-EP390239FDX
CSB-BP390239FDX
CSB-MP390239FDX
CSB-EP390239FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP390240FDX
CSB-EP390240FDX
CSB-BP390240FDX
CSB-MP390240FDX
CSB-EP390240FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis 30S ribosomal protein S18 (rpsR) CSB-YP390241FDX
CSB-EP390241FDX
CSB-BP390241FDX
CSB-MP390241FDX
CSB-EP390241FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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