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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lepidium virginicum Putative membrane protein ycf1 (ycf1-A), partial CSB-YP390642LLP
CSB-EP390642LLP
CSB-BP390642LLP
CSB-MP390642LLP
CSB-EP390642LLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP390643LLU
CSB-EP390643LLU
CSB-BP390643LLU
CSB-MP390643LLU
CSB-EP390643LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP390644LLU
CSB-EP390644LLU
CSB-BP390644LLU
CSB-MP390644LLU
CSB-EP390644LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Photosystem II D2 protein (psbD), partial CSB-YP390645LLU1
CSB-EP390645LLU1
CSB-BP390645LLU1
CSB-MP390645LLU1
CSB-EP390645LLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP390646LLU
CSB-EP390646LLU
CSB-BP390646LLU
CSB-MP390646LLU
CSB-EP390646LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP390647LLU1
CSB-EP390647LLU1
CSB-BP390647LLU1
CSB-MP390647LLU1
CSB-EP390647LLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP390648LLU
CSB-EP390648LLU
CSB-BP390648LLU
CSB-MP390648LLU
CSB-EP390648LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP390649LLU
CSB-EP390649LLU
CSB-BP390649LLU
CSB-MP390649LLU
CSB-EP390649LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP390650LLU1
CSB-EP390650LLU1
CSB-BP390650LLU1
CSB-MP390650LLU1
CSB-EP390650LLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP390651LLU
CSB-EP390651LLU
CSB-BP390651LLU
CSB-MP390651LLU
CSB-EP390651LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP390652LLU
CSB-EP390652LLU
CSB-BP390652LLU
CSB-MP390652LLU
CSB-EP390652LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP390653LLU
CSB-EP390653LLU
CSB-BP390653LLU
CSB-MP390653LLU
CSB-EP390653LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Putative uncharacterized protein ycf15 (ycf15-A) CSB-YP390654LLU
CSB-EP390654LLU
CSB-BP390654LLU
CSB-MP390654LLU
CSB-EP390654LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic, partial CSB-YP390655LLU
CSB-EP390655LLU
CSB-BP390655LLU
CSB-MP390655LLU
CSB-EP390655LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima Photosystem I iron-sulfur center (psaC) CSB-YP390656LLU
CSB-EP390656LLU
CSB-BP390656LLU
CSB-MP390656LLU
CSB-EP390656LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lobularia maritima NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP390657LLU
CSB-EP390657LLU
CSB-BP390657LLU
CSB-MP390657LLU
CSB-EP390657LLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale Photosystem II reaction center protein I (psbI), partial CSB-YP390658NEV
CSB-EP390658NEV
CSB-BP390658NEV
CSB-MP390658NEV
CSB-EP390658NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP390659NEV1
CSB-EP390659NEV1
CSB-BP390659NEV1
CSB-MP390659NEV1
CSB-EP390659NEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP390660NEV
CSB-EP390660NEV
CSB-BP390660NEV
CSB-MP390660NEV
CSB-EP390660NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nasturtium officinale 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP390661NEV
CSB-EP390661NEV
CSB-BP390661NEV
CSB-MP390661NEV
CSB-EP390661NEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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