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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Magnaporthe oryzae Nascent polypeptide-associated complex subunit beta (EGD1) CSB-YP390702MPM
CSB-EP390702MPM
CSB-BP390702MPM
CSB-MP390702MPM
CSB-EP390702MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Histone H3 (HHT1) CSB-YP390703MPM
CSB-EP390703MPM
CSB-BP390703MPM
CSB-MP390703MPM
CSB-EP390703MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae RNA polymerase II holoenzyme cyclin-like subunit (SSN8) CSB-YP390704MPM
CSB-EP390704MPM
CSB-BP390704MPM
CSB-MP390704MPM
CSB-EP390704MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Protein transport protein SEC13 (SEC13) CSB-YP390705MPM
CSB-EP390705MPM
CSB-BP390705MPM
CSB-MP390705MPM
CSB-EP390705MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Vacuolar protein sorting/targeting protein 10 (VPS10), partial CSB-YP390706MPM
CSB-EP390706MPM
CSB-BP390706MPM
CSB-MP390706MPM
CSB-EP390706MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Class E vacuolar protein-sorting machinery protein HSE1 (HSE1), partial CSB-YP390707MPM
CSB-EP390707MPM
CSB-BP390707MPM
CSB-MP390707MPM
CSB-EP390707MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Protein SIP5 (SIP5), partial CSB-YP390708MPM
CSB-EP390708MPM
CSB-BP390708MPM
CSB-MP390708MPM
CSB-EP390708MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Altered inheritance of mitochondria protein 31, mitochondrial (AIM31), partial CSB-YP390709MPM1
CSB-EP390709MPM1
CSB-BP390709MPM1
CSB-MP390709MPM1
CSB-EP390709MPM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Mitochondrial division protein 1 (MDV1), partial CSB-YP390710MPM
CSB-EP390710MPM
CSB-BP390710MPM
CSB-MP390710MPM
CSB-EP390710MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Dicer-like protein 1 (DCL1), partial CSB-YP390711MPM
CSB-EP390711MPM
CSB-BP390711MPM
CSB-MP390711MPM
CSB-EP390711MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae GDP-mannose transporter (VRG4), partial CSB-YP390712MPM
CSB-EP390712MPM
CSB-BP390712MPM
CSB-MP390712MPM
CSB-EP390712MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Probable endonuclease LCL3 (LCL3), partial CSB-YP390713MPM1
CSB-EP390713MPM1
CSB-BP390713MPM1
CSB-MP390713MPM1
CSB-EP390713MPM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Adenylyltransferase and sulfurtransferase UBA4 (UBA4) CSB-YP390714MPM
CSB-EP390714MPM
CSB-BP390714MPM
CSB-MP390714MPM
CSB-EP390714MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Probable Xaa-Pro aminopeptidase MGG_05684 (MGG_05684), partial CSB-YP390715MPM
CSB-EP390715MPM
CSB-BP390715MPM
CSB-MP390715MPM
CSB-EP390715MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreococcus lucimarinus Adenylosuccinate synthetase, chloroplastic (PURA) CSB-YP390716ODL
CSB-EP390716ODL
CSB-BP390716ODL
CSB-MP390716ODL
CSB-EP390716ODL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreococcus lucimarinus Lipoyl synthase, mitochondrial (LIP1) CSB-YP390717ODL
CSB-EP390717ODL
CSB-BP390717ODL
CSB-MP390717ODL
CSB-EP390717ODL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreococcus lucimarinus Arginine biosynthesis bifunctional protein ArgJ, chloroplastic (OSTLU_33128) CSB-YP390718ODL
CSB-EP390718ODL
CSB-BP390718ODL
CSB-MP390718ODL
CSB-EP390718ODL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreococcus lucimarinus Lon protease homolog, mitochondrial (OSTLU_41620, OSTLU_52138), partial CSB-YP390719ODL
CSB-EP390719ODL
CSB-BP390719ODL
CSB-MP390719ODL
CSB-EP390719ODL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP390720PZJ
CSB-EP390720PZJ
CSB-BP390720PZJ
CSB-MP390720PZJ
CSB-EP390720PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 30S ribosomal protein S18 (rpsR) CSB-YP390721PZJ
CSB-EP390721PZJ
CSB-BP390721PZJ
CSB-MP390721PZJ
CSB-EP390721PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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